PMID- 9156554
OWN - NLM
STAT- MEDLINE
DCOM- 19970522
LR  - 20131121
IS  - 0320-9725 (Print)
IS  - 0320-9725 (Linking)
VI  - 61
IP  - 12
DP  - 1996 Dec
TI  - [Selective chemical modification of cytochrome P450scc (CYP11A1) with 
      diiodofluorescein iodoacetamide in the study of the role and topology of 
      interdomain hinge of the hemoprotein molecule].
PG  - 2103-15
AB  - Bovine adrenocortical cytochrome P450scc (CYP11A1) was selectively modified with 
      diiodofluorescein iodoacetamide (DIFIA). Only Cys264 is labeled in the P450 
      polypeptide chain. The modification significantly affected the 
      cholesterol-hydroxylating activity in the reconstituted system containing NADPH, 
      adrenodoxin reductase, adrenodoxin, and soluble or membrane-bound P450scc. The 
      inhibitory effect correlates with decreased affinity of cytochrome P450scc to 
      intermediate electron carrier, adrenodoxin. Cytochrome P450scc is modified in 
      liposomes and the modified membrane-bound protein is cleaved by trypsin forming 
      two large fragments F1 and F2 corresponding to the N- and C-terminal regions of 
      the molecule. The data indicate that the Cys264-containing region of the 
      cytochrome P450scc molecule is exposed to the surface of protein globule, located 
      outside of the membrane, and can participate in protein-protein interactions.
FAU - Chernogolov, A A
AU  - Chernogolov AA
FAU - Schwarz, D
AU  - Schwarz D
FAU - Usanov, S A
AU  - Usanov SA
LA  - rus
PT  - English Abstract
PT  - Journal Article
TT  - Selektivnaia khimicheskaia modifikatsiia tsitokhroma P450scc (CYP11A1) 
      diiodofluorosceiniodoatsetamidom v issledovanii roli i topologii mezhdomennogo 
      uchastka molekuly gemoproteina.
PL  - Russia (Federation)
TA  - Biokhimiia
JT  - Biokhimiia (Moscow, Russia)
JID - 0372667
RN  - 0 (Fluoresceins)
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Liposomes)
RN  - 0 (Membrane Proteins)
RN  - 0 (Proteolipids)
RN  - 0 (diiodofluorescein iodoacetamide)
RN  - 0 (proteoliposomes)
RN  - 12687-22-8 (Adrenodoxin)
RN  - EC 1.14.15.6 (Cholesterol Side-Chain Cleavage Enzyme)
RN  - K848JZ4886 (Cysteine)
RN  - ZRH8M27S79 (Iodoacetamide)
SB  - IM
MH  - Adrenal Cortex/enzymology
MH  - Adrenodoxin/chemistry
MH  - Animals
MH  - Cattle
MH  - Cholesterol Side-Chain Cleavage Enzyme/*chemistry
MH  - Cysteine/chemistry
MH  - *Fluoresceins
MH  - *Fluorescent Dyes
MH  - Iodoacetamide/*analogs & derivatives
MH  - Liposomes/chemistry
MH  - Membrane Proteins/*chemistry
MH  - Proteolipids/chemistry
EDAT- 1996/12/01 00:00
MHDA- 1996/12/01 00:01
CRDT- 1996/12/01 00:00
PHST- 1996/12/01 00:00 [pubmed]
PHST- 1996/12/01 00:01 [medline]
PHST- 1996/12/01 00:00 [entrez]
PST - ppublish
SO  - Biokhimiia. 1996 Dec;61(12):2103-15.