PMID- 9158037
OWN - NLM
STAT- MEDLINE
DCOM- 19970606
LR  - 20190713
IS  - 0041-1337 (Print)
IS  - 0041-1337 (Linking)
VI  - 63
IP  - 9
DP  - 1997 May 15
TI  - Human cytomegalovirus does not induce human leukocyte antigen class II expression 
      on arterial endothelial cells.
PG  - 1366-9
AB  - Human cytomegalovirus (CMV) has been associated with allograft rejection and, in 
      particular, with transplant-associated arteriosclerosis. However, the role CMV 
      plays in the development of transplant-associated arteriosclerosis remains 
      unclear. CMV can infect the endothelium, the interface between allograft tissue 
      and the host immune cells, but the direct induction of endothelial human 
      leukocyte antigen (HLA) class II by CMV remains controversial. Our previous 
      studies with venous endothelial cells (EC) have shown that CMV does not directly 
      induce this antigen on infected EC and, furthermore, renders these cells 
      refractory to interferon (IFN)-gamma induction. However, questions have arisen 
      regarding the relevance of these findings to arterial endothelia. Thus, we have 
      extended these studies to determine whether similar interactions occur in 
      arterial EC. EC derived from human coronary artery, aorta, and umbilical artery 
      were assayed by immunofluorescence flow cytometry and dual immunohistochemical 
      staining following IFN-gamma treatment and/or inoculation with CMV. Data 
      generated by these experiments demonstrate that regardless of vascular origin: 
      (1) CMV does not directly induce endothelial surface or cytoplasmic HLA class II, 
      and (2) although uninfected arterial EC are HLA class II inducible by IFN-gamma, 
      infected cells are completely refractory to this effect. These results suggest 
      that CMV-mediated inhibition of HLA class II expression is a phenomenon shared by 
      human arterial and venous endothelia of both fetal and adult origin.
FAU - Knight, D A
AU  - Knight DA
AD  - Department of Pathology, The Ohio State University College of Medicine, Columbus 
      43210-1238, USA.
FAU - Waldman, W J
AU  - Waldman WJ
FAU - Sedmak, D D
AU  - Sedmak DD
LA  - eng
GR  - AI29002/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Transplantation
JT  - Transplantation
JID - 0132144
RN  - 0 (Histocompatibility Antigens Class II)
SB  - IM
MH  - Adult
MH  - Arteries/cytology/metabolism/virology
MH  - Arteriosclerosis/etiology
MH  - Cells, Cultured
MH  - *Cytomegalovirus
MH  - Cytomegalovirus Infections/immunology/*metabolism
MH  - Endothelium, Vascular/*metabolism/*virology
MH  - Histocompatibility Antigens Class II/*biosynthesis
MH  - Humans
MH  - Transplantation/adverse effects
EDAT- 1997/05/15 00:00
MHDA- 1997/05/15 00:01
CRDT- 1997/05/15 00:00
PHST- 1997/05/15 00:00 [pubmed]
PHST- 1997/05/15 00:01 [medline]
PHST- 1997/05/15 00:00 [entrez]
AID - 10.1097/00007890-199705150-00030 [doi]
PST - ppublish
SO  - Transplantation. 1997 May 15;63(9):1366-9. doi: 10.1097/00007890-199705150-00030.