PMID- 9158941
OWN - NLM
STAT- MEDLINE
DCOM- 19970710
LR  - 20061115
IS  - 0015-5500 (Print)
IS  - 0015-5500 (Linking)
VI  - 42
IP  - 6
DP  - 1996
TI  - Fluorescence in situ hybridization (FISH) in cytogenetics of leukemia.
PG  - 311-4
AB  - The principle of the fluorescence in situ hybridization (FISH) method is in the 
      base pairing of the DNA probe to complementary sequences in the studied specimen. 
      The hybridization of specific DNA or RNA probes to the cellular targets attached 
      to the microscopic slides is widely used for the identification of chromosomal 
      translocations, deletions, amplifications of specific genes, and chromosome 
      number changes in mitotic and/or interphase cells. The use of FISH with the 
      modifications of the basic method meant a breakthrough in detection and diagnosis 
      of human malignancies. During the last tow years FISH was used in our laboratory 
      for: (a) identification of constitutive and acquired numerical and structural 
      chromosomal abnormalities; (b) detection of minimal residual disease or early 
      relapse in patients treated for leukemia by bone marrow transplantation (BMT) 
      and/or chemotherapy; (c) determination of the cytogenetic pattern of non-dividing 
      or terminally differentiated cells. To confirm the structural rearrangements 
      found by the classical G-banding technique, the whole chromosome painting probes 
      which hybridize to multiple chromosomal sequences were used. The alpha-satellite 
      DNA probes which detect centromeric repetitive sequences were utilized for 
      determining the numerical and sex chromosome changes. Specific unique chromosomal 
      sequences which can confirm all chromosomal rearrangements, i.e., deletions, 
      translocations or inversions with the corresponding breakpoints were introduced 
      for specific cases. Recently, every chromosomal translocation, deletion and any 
      other structural or numerical change found by conventional cytogenetic analysis 
      in the bone marrow cells of the patients with leukemia has been verified in our 
      laboratory by FISH. The results of this study showed that FISH is more efficient 
      than conventional cytogenetics in detecting residual malignant cells. For 
      chromosomal rearrangements FISH is an extremely sensitive method which not only 
      verifies but also interprets with more precision the findings of classical 
      cytogenetics.
FAU - Michalova, K
AU  - Michalova K
AD  - 3rd Medical Department, General Faculty Hospital, Prague, Czech Republic.
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Review
PL  - Czech Republic
TA  - Folia Biol (Praha)
JT  - Folia biologica
JID - 0234640
RN  - 0 (DNA Probes)
RN  - 0 (DNA, Satellite)
RN  - 0 (RNA Probes)
SB  - IM
MH  - *Chromosome Aberrations
MH  - Cytogenetics/methods
MH  - DNA Probes
MH  - DNA, Satellite/analysis/genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Leukemia/*genetics/pathology
MH  - RNA Probes
RF  - 13
EDAT- 1996/01/01 00:00
MHDA- 1996/01/01 00:01
CRDT- 1996/01/01 00:00
PHST- 1996/01/01 00:00 [pubmed]
PHST- 1996/01/01 00:01 [medline]
PHST- 1996/01/01 00:00 [entrez]
PST - ppublish
SO  - Folia Biol (Praha). 1996;42(6):311-4.