PMID- 9159173
OWN - NLM
STAT- MEDLINE
DCOM- 19970619
LR  - 20190501
IS  - 0027-8424 (Print)
IS  - 1091-6490 (Electronic)
IS  - 0027-8424 (Linking)
VI  - 94
IP  - 11
DP  - 1997 May 27
TI  - The neuron-restrictive silencer element: a dual enhancer/silencer crucial for 
      patterned expression of a nicotinic receptor gene in the brain.
PG  - 5906-11
AB  - The neuron-restrictive silencer element (NRSE) has been identified in several 
      neuronal genes and confers neuron specificity by silencing transcription in 
      nonneuronal cells. NRSE is present in the promoter of the neuronal nicotinic 
      acetylcholine receptor beta2-subunit gene that determines its neuron-specific 
      expression in the nervous system. Using transgenic mice, we show that NRSE may 
      either silence or enhance transcription depending on the cellular context within 
      the nervous system. In vitro in neuronal cells, NRSE activates transcription of 
      synthetic promoters when located downstream in the 5' untranslated region, or at 
      less than 50 bp upstream from the TATA box, but switches to a silencer when 
      located further upstream. In contrast, in nonneuronal cells NRSE always functions 
      as a silencer. Antisense RNA inhibition shows that the NRSE-binding protein REST 
      contributes to the activation of transcription in neuronal cells.
FAU - Bessis, A
AU  - Bessis A
AD  - Neurobiologie Moleculaire, UA CNRS D1284, Departement des Biotechnologies, 
      Institut Pasteur 25/28 rue du Dr Roux, 75724 Paris Cedex 15, France. 
      abisses@pasteur.fr
FAU - Champtiaux, N
AU  - Champtiaux N
FAU - Chatelin, L
AU  - Chatelin L
FAU - Changeux, J P
AU  - Changeux JP
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (DNA Primers)
RN  - 0 (Oligodeoxyribonucleotides)
RN  - 0 (Receptors, Nicotinic)
RN  - 0 (Recombinant Fusion Proteins)
RN  - EC 3.2.1.23 (beta-Galactosidase)
SB  - IM
MH  - Animals
MH  - Brain/*metabolism
MH  - Cell Line
MH  - DNA Primers
MH  - *Enhancer Elements, Genetic
MH  - Mice
MH  - Mice, Transgenic
MH  - Neuroblastoma
MH  - Neurons/*metabolism
MH  - Oligodeoxyribonucleotides
MH  - Organ Specificity
MH  - Point Mutation
MH  - Polymerase Chain Reaction
MH  - *Promoter Regions, Genetic
MH  - Receptors, Nicotinic/*biosynthesis/genetics
MH  - Recombinant Fusion Proteins/biosynthesis
MH  - *Regulatory Sequences, Nucleic Acid
MH  - TATA Box
MH  - Transfection
MH  - Tumor Cells, Cultured
MH  - beta-Galactosidase/biosynthesis/genetics
PMC - PMC20879
EDAT- 1997/05/27 00:00
MHDA- 1997/05/27 00:01
PMCR- 1997/11/27
CRDT- 1997/05/27 00:00
PHST- 1997/05/27 00:00 [pubmed]
PHST- 1997/05/27 00:01 [medline]
PHST- 1997/05/27 00:00 [entrez]
PHST- 1997/11/27 00:00 [pmc-release]
AID - 0883 [pii]
AID - 10.1073/pnas.94.11.5906 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 1997 May 27;94(11):5906-11. doi: 
      10.1073/pnas.94.11.5906.