PMID- 9160386
OWN - NLM
STAT- MEDLINE
DCOM- 19970716
LR  - 20190905
IS  - 0197-3851 (Print)
IS  - 0197-3851 (Linking)
VI  - 17
IP  - 4
DP  - 1997 Apr
TI  - Prenatal detection of chromosome aneuploidies by fluorescence in situ 
      hybridization: experience with 2000 uncultured amniotic fluid samples in a 
      prospective preclinical trial.
PG  - 333-41
AB  - Successful rapid prenatal detection of selected numerical chromosome 
      abnormalities by using fluorescence in situ hybridization (FISH) on uncultured 
      amniotic fluid samples has been described by Klinger et al. (1992) and Ward et 
      al. (1993, 1997). Using essentially the same FISH protocol and identical probes 
      specific for chromosomes 21, 18, 13, X, and Y, we prospectively compared the 
      results of FISH and conventional cytogenetics on 2000 amniotic fluid cell 
      samples. The 1-day FISH assay yielded discrete differences in the signal profiles 
      between cytogenetically disomic, i.e., normal, and trisomic samples. Due to 
      intermittent absent Y-signals, the assay differentiated less well between samples 
      with cytogenetically normal and abnormal sex chromosome complements. The assay 
      efficiency, and thus the clinical utility, was affected by (1) unsuccessful 
      hybridizations (7 per cent of all hybridizations), (2) hybridizations with less 
      than 50 scorable nuclei (19 per cent of all hybridizations), and (3) visibly 
      contaminated samples with possible maternal cell contamination (14 per cent of 
      all samples). As a result, we were not able to reproduce the results of Klinger 
      et al. (1992) and Ward et al. (1993, 1997).
FAU - Bryndorf, T
AU  - Bryndorf T
AD  - Chromosome Laboratory, Juliane Marie Center, Rigshospital, University of 
      Copenhagen, Denmark. t-bryndorf@dk-online.dk
FAU - Christensen, B
AU  - Christensen B
FAU - Vad, M
AU  - Vad M
FAU - Parner, J
AU  - Parner J
FAU - Brocks, V
AU  - Brocks V
FAU - Philip, J
AU  - Philip J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Prenat Diagn
JT  - Prenatal diagnosis
JID - 8106540
SB  - IM
MH  - Amniotic Fluid/*cytology
MH  - *Aneuploidy
MH  - Chromosomes, Human, Pair 13
MH  - Chromosomes, Human, Pair 18
MH  - Chromosomes, Human, Pair 21
MH  - Female
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Pregnancy
MH  - *Prenatal Diagnosis
MH  - Prospective Studies
MH  - Sex Chromosomes
EDAT- 1997/04/01 00:00
MHDA- 2000/06/20 09:00
CRDT- 1997/04/01 00:00
PHST- 1997/04/01 00:00 [pubmed]
PHST- 2000/06/20 09:00 [medline]
PHST- 1997/04/01 00:00 [entrez]
AID - 10.1002/(SICI)1097-0223(199704)17:4<333::AID-PD76>3.0.CO;2-# [pii]
AID - 10.1002/(sici)1097-0223(199704)17:4<333::aid-pd76>3.0.co;2-# [doi]
PST - ppublish
SO  - Prenat Diagn. 1997 Apr;17(4):333-41. doi: 
      10.1002/(sici)1097-0223(199704)17:4<333::aid-pd76>3.0.co;2-#.