PMID- 9162055
OWN - NLM
STAT- MEDLINE
DCOM- 19970627
LR  - 20210209
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 272
IP  - 22
DP  - 1997 May 30
TI  - Differential activity of progesterone and glucocorticoid receptors on mouse 
      mammary tumor virus templates differing in chromatin structure.
PG  - 14227-35
AB  - In vivo, transcription factors interact with promoters having complex 
      nucleoprotein structures. The transiently expressed progesterone receptor (PR) 
      efficiently activates a transfected mouse mammary tumor virus (MMTV) promoter but 
      is a poor activator of the MMTV promoter when it acquires an ordered chromatin 
      structure as an endogenous, replicating gene. We show that the deficiency in PR 
      activity is not due to insufficient expression of either B or A isoforms or 
      competition between the two types of MMTV templates. Rather, this deficiency 
      reflects an inability to induce the chromatin remodeling event that is required 
      for activation of the replicated MMTV template. To determine whether this 
      characteristic is common to transiently expressed steroid receptors or specific 
      to the PR, we examined the activity of transiently expressed glucocorticoid (GR) 
      receptor. Unlike the PR, the transiently expressed GR is an effective activator 
      of both MMTV templates and efficiently induces the necessary chromatin remodeling 
      event at the replicated template. These results indicate that the GR and PR have 
      unique requirements for activation of promoters with ordered chromatin structure. 
      These differences may provide a mechanism for establishing target gene 
      specificity in vivo for steroid receptors that recognize and bind to identical 
      DNA sequences.
FAU - Smith, C L
AU  - Smith CL
AD  - Laboratory of Receptor Biology and Gene Expression, National Cancer Institute, 
      National Institutes of Health, Bethesda, Maryland 20892-5055, USA.
FAU - Htun, H
AU  - Htun H
FAU - Wolford, R G
AU  - Wolford RG
FAU - Hager, G L
AU  - Hager GL
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Chromatin)
RN  - 0 (Receptors, Glucocorticoid)
RN  - 0 (Receptors, Progesterone)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Cell Line
MH  - Chromatin/*genetics/ultrastructure
MH  - *Gene Expression Regulation, Viral
MH  - Mammary Tumor Virus, Mouse/*physiology
MH  - Mice
MH  - Molecular Sequence Data
MH  - Promoter Regions, Genetic/genetics
MH  - Receptors, Glucocorticoid/*physiology
MH  - Receptors, Progesterone/*physiology
MH  - Transfection
EDAT- 1997/05/30 00:00
MHDA- 1997/05/30 00:01
CRDT- 1997/05/30 00:00
PHST- 1997/05/30 00:00 [pubmed]
PHST- 1997/05/30 00:01 [medline]
PHST- 1997/05/30 00:00 [entrez]
AID - S0021-9258(19)62539-1 [pii]
AID - 10.1074/jbc.272.22.14227 [doi]
PST - ppublish
SO  - J Biol Chem. 1997 May 30;272(22):14227-35. doi: 10.1074/jbc.272.22.14227.