PMID- 9162196
OWN - NLM
STAT- MEDLINE
DCOM- 19970527
LR  - 20071115
IS  - 1045-2257 (Print)
IS  - 1045-2257 (Linking)
VI  - 16
IP  - 1
DP  - 1996 May
TI  - Probes for hidden hyperdiploidy in acute lymphoblastic leukaemia.
PG  - 40-5
AB  - The detection of hyperdiploidy (clones with >46 chromosomes) in the bone marrow 
      of patients with acute lymphoblastic leukaemia (ALL) is important because of the 
      prognostic impact of this finding. The high hyperdiploid (HeH) subgroup with 
      51-68 chromosomes is associated with the best outcome, followed by the low 
      hyperdiploid (HeL) subgroup with 47-50 chromosomes and the triploid/tetraploid 
      (TT) subgroup with >68 chromosomes, which do less well. We present a strategy for 
      the use of fluorescence in situ hybridization (FISH) with chromosome-specific 
      probes to detect hyperdiploidy in interphase cells and to assign cases to a 
      ploidy subgroup. By using a model population of 252 cases, it was seen that ten 
      chromosomes (X, 4, 6, 8, 10, 14, 16, 18, 20, and 21) used in particular 
      combinations and applied in a step-wise manner enabled the detection of 94% of 
      hyperdiploid cases and gave an accurate prediction of ploidy subgroup in 96% of 
      these cases. The detection and classification of each case required the use of 
      four to six probes over two or three steps. Confirmation that this strategy will 
      achieve this level of detection in other hyperdiploid populations was 
      demonstrated by using 250 published karyotypes. This strategy has an application 
      in detecting missing or hidden hyperdiploid cases among cases with failed or 
      normal cytogenetics.
FAU - Moorman, A V
AU  - Moorman AV
AD  - Haematology Department, Royal Free Hospital School of Medicine, London, United 
      Kingdom.
FAU - Clark, R
AU  - Clark R
FAU - Farrell, D M
AU  - Farrell DM
FAU - Hawkins, J M
AU  - Hawkins JM
FAU - Martineau, M
AU  - Martineau M
FAU - Secker-Walker, L M
AU  - Secker-Walker LM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Genes Chromosomes Cancer
JT  - Genes, chromosomes & cancer
JID - 9007329
SB  - IM
MH  - *Chromosome Aberrations
MH  - *Diploidy
MH  - Humans
MH  - Karyotyping
MH  - Precursor Cell Lymphoblastic Leukemia-Lymphoma/*genetics
EDAT- 1996/05/01 00:00
MHDA- 2000/06/20 09:00
CRDT- 1996/05/01 00:00
PHST- 1996/05/01 00:00 [pubmed]
PHST- 2000/06/20 09:00 [medline]
PHST- 1996/05/01 00:00 [entrez]
AID - 10.1002/(SICI)1098-2264(199605)16:1<40::AID-GCC6>3.0.CO;2-3 [pii]
AID - 10.1002/(SICI)1098-2264(199605)16:1<40::AID-GCC6>3.0.CO;2-3 [doi]
PST - ppublish
SO  - Genes Chromosomes Cancer. 1996 May;16(1):40-5. doi: 
      10.1002/(SICI)1098-2264(199605)16:1<40::AID-GCC6>3.0.CO;2-3.