PMID- 9162738
OWN - NLM
STAT- MEDLINE
DCOM- 19970529
LR  - 20190909
IS  - 0303-6987 (Print)
IS  - 0303-6987 (Linking)
VI  - 24
IP  - 2
DP  - 1997 Feb
TI  - Numerical aberrations of chromosome 7 detected in 15 microns paraffin-embedded 
      tissue sections of primary cutaneous melanomas by fluorescence in situ 
      hybridization and confocal laser scanning microscopy.
PG  - 70-5
AB  - Fluorescence in situ hybridization (FISH) to sections of formalin-fixed 
      paraffin-embedded archival tissues allows the detection of gene or chromosome 
      copy number changes in interphase cell nuclei within the histological context and 
      thus may be of particular interest in tumor pathology. In this report, we 
      describe the application of FISH to thick (15 microns) paraffin sections of 7 
      primary cutaneous malignant melanomas. A chromosome 7-specific centromeric DNA 
      probe was used to detect numerical aberrations of chromosome 7. By optical 
      sectioning using confocal laser scanning microscopy (CLSM) only complete, uncut 
      interphase cell nuclei were scored. The mean percentage (+/- SEM) of melanoma 
      cell nuclei with three hybridization spots was (20.7 +/- 2.8)%; (6.8 +/- 1.0)% of 
      nuclei showed one spot and (5.0 +/- 1.2)% four or more spots. The frequency 
      distribution of spot numbers among melanoma cell nuclei and normal keratinocyte 
      nuclei was significantly different (chi(2) = 176.8, df = 5, p < 0.001). Trisomy 7 
      was detected in all 7 cases analyzed, mostly associated with monosomy 7 or 
      polysomy 7. The approach used in our study and the data obtained could be useful 
      for further studies designed to investigate a possible involvement of chromosome 
      7 in melanocytic tumor progression.
FAU - D'Alessandro, I
AU  - D'Alessandro I
AD  - Department of Dermatology, University of L'Aquila, Italy.
FAU - Zitzelsberger, H
AU  - Zitzelsberger H
FAU - Hutzler, P
AU  - Hutzler P
FAU - Lehmann, L
AU  - Lehmann L
FAU - Braselmann, H
AU  - Braselmann H
FAU - Chimenti, S
AU  - Chimenti S
FAU - Hofler, H
AU  - Hofler H
LA  - eng
PT  - Clinical Trial
PT  - Journal Article
PL  - United States
TA  - J Cutan Pathol
JT  - Journal of cutaneous pathology
JID - 0425124
SB  - IM
MH  - Adult
MH  - Aged
MH  - *Chromosome Aberrations
MH  - *Chromosomes, Human, Pair 7
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Melanoma/*genetics
MH  - Microscopy, Confocal
MH  - Paraffin Embedding
MH  - Skin Neoplasms/*genetics
EDAT- 1997/02/01 00:00
MHDA- 1997/02/01 00:01
CRDT- 1997/02/01 00:00
PHST- 1997/02/01 00:00 [pubmed]
PHST- 1997/02/01 00:01 [medline]
PHST- 1997/02/01 00:00 [entrez]
AID - 10.1111/j.1600-0560.1997.tb01099.x [doi]
PST - ppublish
SO  - J Cutan Pathol. 1997 Feb;24(2):70-5. doi: 10.1111/j.1600-0560.1997.tb01099.x.