PMID- 9165485
OWN - NLM
STAT- MEDLINE
DCOM- 19970718
LR  - 20191102
IS  - 1044-7393 (Print)
IS  - 1044-7393 (Linking)
VI  - 30
IP  - 3
DP  - 1997 Apr
TI  - The effects of oxidative stress on in vivo brain GSH turnover in young and mature 
      mice.
PG  - 187-97
AB  - Glutathione (GSH) synthetase activities and GSH turnover rates were examined 
      during severe oxidative stress in the mouse brain as induced by 
      t-butylhydroperoxide (t-BuOOH). Brain GSH synthetase activities in 8-mo-old mice 
      in the cortex, striatum, thalamus, hippocampus, midbrain, and cerebellum were 
      found to increase following t-BuOOH treatment. The effect of GSH synthesis on 
      brain GSH turnover rates for 2- and 8-mo-old mice were determined after 
      intracerebroventricular (icv) injection of [35S]cysteine. Rate constants for GSH 
      turnover were determined by least-squares iterative minimization from the 
      specific activity data from 20 min to 108 h after [35S]cysteine administration. 
      GSH and glutathione disulfide (GSSG) specific activities were determined after 
      separation by high-pressure liquid chromatography (HPLC). The half-life of GSH in 
      the 2-mo-old mouse was 59.5 h and in the 8-mo-old mouse was 79.1 h. In summary, 
      defense mechanisms against oxidative stress in the brain differ with age. Young 
      mice can increase the cellular availability of GSH, whereas mature mice can 
      increase GSH synthetase activity during oxidative stress. These differences make 
      mature mice more susceptible to brain oxidative damage.
FAU - Chang, M L
AU  - Chang ML
AD  - Department of Molecular Pharmacology and Toxicology, School of Pharmacy, 
      University of Southern California, Los Angeles 90033, USA.
FAU - Klaidman, L K
AU  - Klaidman LK
FAU - Adams, J D Jr
AU  - Adams JD Jr
LA  - eng
GR  - NS29442/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Mol Chem Neuropathol
JT  - Molecular and chemical neuropathology
JID - 8910358
RN  - 0 (Sulfur Radioisotopes)
RN  - EC 6.3.2.3 (Glutathione Synthase)
RN  - GAN16C9B8O (Glutathione)
SB  - IM
MH  - Aging/*metabolism
MH  - Animals
MH  - Brain/enzymology/*metabolism
MH  - Glutathione/*metabolism
MH  - Glutathione Synthase/metabolism
MH  - Male
MH  - Mice
MH  - Mice, Inbred Strains
MH  - Oxidative Stress/*physiology
MH  - Sulfur Radioisotopes/metabolism
MH  - Time Factors
EDAT- 1997/04/01 00:00
MHDA- 2001/03/28 10:01
CRDT- 1997/04/01 00:00
PHST- 1997/04/01 00:00 [pubmed]
PHST- 2001/03/28 10:01 [medline]
PHST- 1997/04/01 00:00 [entrez]
AID - 10.1007/BF02815097 [doi]
PST - ppublish
SO  - Mol Chem Neuropathol. 1997 Apr;30(3):187-97. doi: 10.1007/BF02815097.