PMID- 9166744
OWN - NLM
STAT- MEDLINE
DCOM- 19970617
LR  - 20190630
IS  - 0022-3042 (Print)
IS  - 0022-3042 (Linking)
VI  - 68
IP  - 6
DP  - 1997 Jun
TI  - Koningic acid (a potent glyceraldehyde-3-phosphate dehydrogenase 
      inhibitor)-induced fragmentation and condensation of DNA in NG108-15 cells.
PG  - 2493-9
AB  - We examined nitric oxide (NO)-induced cell death in NG108-15 cells using NO 
      donors. Both sodium nitroprusside (SNP) and S-nitroso-N-acetylpenicillamine 
      caused lactate dehydrogenase (LDH) leakage from NG108-15 cells. NO is known to 
      increase the amount of radioisotopic labeled glyceraldehyde-3-phosphate 
      dehydrogenase (GAPDH) in the presence of [32P]NAD and to inhibit the enzyme 
      activity. To clarify the relationship between the NO-induced inhibition of GAPDH 
      activity and cell death, we studied the effect of koningic acid (KA), a potent 
      selective inhibitor of GAPDH. Both SNP and KA elicited LDH leakage, chromosomal 
      condensation, and fragmentation of nuclei in NG108-15 cells. Gel electrophoretic 
      analysis of cellular DNA extracted from SNP- and KA-treated cells revealed the 
      internucleosomal DNA fragmentation typical of apoptosis in these cultures. The 
      results suggested that in NG108-15 cells, (a) the inhibition of GAPDH activity 
      results in apoptosis and (b) SNP-induced cell death is partly due to the 
      NO-induced inhibition of GAPDH, perhaps by stimulating the binding of NAD to 
      GAPDH.
FAU - Nakazawa, M
AU  - Nakazawa M
AD  - Department of Pharmacology, Faculty of Pharmaceutical Sciences, Hokkaido 
      University, Sapporo, Japan.
FAU - Uehara, T
AU  - Uehara T
FAU - Nomura, Y
AU  - Nomura Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Neurochem
JT  - Journal of neurochemistry
JID - 2985190R
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (DNA, Neoplasm)
RN  - 0 (Sesquiterpenes)
RN  - 0 (Sulfhydryl Reagents)
RN  - 169D1260KM (Nitroprusside)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - 57710-57-3 (heptelidic acid)
RN  - EC 1.1.1.27 (L-Lactate Dehydrogenase)
RN  - EC 1.2.1.- (Glyceraldehyde-3-Phosphate Dehydrogenases)
SB  - IM
MH  - Animals
MH  - Anti-Bacterial Agents/*pharmacology
MH  - Apoptosis/drug effects
MH  - Cell Nucleus/drug effects
MH  - DNA Fragmentation/*drug effects
MH  - DNA, Neoplasm/*drug effects
MH  - Glioma
MH  - Glyceraldehyde-3-Phosphate Dehydrogenases/antagonists & inhibitors/metabolism
MH  - Hybrid Cells/cytology/drug effects/enzymology
MH  - L-Lactate Dehydrogenase/metabolism
MH  - Mice
MH  - Neuroblastoma
MH  - Nitric Oxide/metabolism
MH  - Nitroprusside/pharmacology
MH  - Rats
MH  - Sesquiterpenes/pharmacology
MH  - Sulfhydryl Reagents/pharmacology
EDAT- 1997/06/01 00:00
MHDA- 1997/06/01 00:01
CRDT- 1997/06/01 00:00
PHST- 1997/06/01 00:00 [pubmed]
PHST- 1997/06/01 00:01 [medline]
PHST- 1997/06/01 00:00 [entrez]
AID - 10.1046/j.1471-4159.1997.68062493.x [doi]
PST - ppublish
SO  - J Neurochem. 1997 Jun;68(6):2493-9. doi: 10.1046/j.1471-4159.1997.68062493.x.