PMID- 9168124
OWN - NLM
STAT- MEDLINE
DCOM- 19970616
LR  - 20190707
IS  - 0378-1119 (Print)
IS  - 0378-1119 (Linking)
VI  - 189
IP  - 2
DP  - 1997 Apr 21
TI  - The mouse uracil-DNA glycosylase gene: isolation of cDNA and genomic clones and 
      mapping ung to mouse chromosome 5.
PG  - 175-81
AB  - Uracil-DNA glycosylase (UDG) is the enzyme responsible for the first step in the 
      base-excision repair pathway that specifically removes uracil from DNA. Here we 
      report the isolation of the cDNA and genomic clones for the mouse uracil-DNA 
      glycosylase gene (ung) homologous to the major placental uracil-DNA glycosylase 
      gene (UNG) of humans. The complete characterization of the genomic organization 
      of the mouse uracil-DNA glycosylase gene shows that the entire mRNA coding region 
      for the 1.83-kb cDNA of the mouse ung gene is contained in an 8.2-kb SstI genomic 
      fragment which includes six exons and five introns. The cDNA encodes a predicted 
      uracil-DNA glycosylase (UDG) protein of 295 amino acids (33 kDa) that is highly 
      similar to a group of UDGs that have been isolated from a wide variety of 
      organisms. The mouse ung gene has been mapped to mouse chromosome 5 using 
      fluorescence in situ hybridization (FISH).
FAU - Svendsen, P C
AU  - Svendsen PC
AD  - Department of Medical Biochemistry, University of Calgary, Health Sciences 
      Centre, Alberta, Canada.
FAU - Yee, H A
AU  - Yee HA
FAU - Winkfein, R J
AU  - Winkfein RJ
FAU - van de Sande, J H
AU  - van de Sande JH
LA  - eng
SI  - GENBANK/U55040
SI  - GENBANK/U55041
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Gene
JT  - Gene
JID - 7706761
RN  - 0 (DNA, Complementary)
RN  - EC 3.2.2.- (DNA Glycosylases)
RN  - EC 3.2.2.- (N-Glycosyl Hydrolases)
RN  - EC 3.2.2.- (Uracil-DNA Glycosidase)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - *Chromosome Mapping
MH  - Cloning, Molecular
MH  - *DNA Glycosylases
MH  - DNA, Complementary/*isolation & purification
MH  - Exons
MH  - *Genes
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Introns
MH  - Mice
MH  - Molecular Sequence Data
MH  - N-Glycosyl Hydrolases/chemistry/*genetics/*isolation & purification
MH  - Protein Binding/genetics
MH  - Repetitive Sequences, Nucleic Acid
MH  - Sequence Analysis, DNA
MH  - Transcription, Genetic
MH  - Uracil-DNA Glycosidase
EDAT- 1997/04/21 00:00
MHDA- 1997/04/21 00:01
CRDT- 1997/04/21 00:00
PHST- 1997/04/21 00:00 [pubmed]
PHST- 1997/04/21 00:01 [medline]
PHST- 1997/04/21 00:00 [entrez]
AID - S0378-1119(96)00797-4 [pii]
AID - 10.1016/s0378-1119(96)00797-4 [doi]
PST - ppublish
SO  - Gene. 1997 Apr 21;189(2):175-81. doi: 10.1016/s0378-1119(96)00797-4.