PMID- 9168973
OWN - NLM
STAT- MEDLINE
DCOM- 19970624
LR  - 20161124
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 234
IP  - 1
DP  - 1997 May 8
TI  - Sphingosine-1-phosphate mobilizes intracellular calcium and activates 
      transcription factor NF-kappa B in U937 cells.
PG  - 121-4
AB  - Sphingosine-1-phosphate (SPP), a metabolite of sphingolipids, has been implicated 
      as a second messenger in cell growth regulation and signal transduction via 
      calcium mobilization from internal stores. This study shows that SPP mobilizes 
      intracellular calcium in U937 cells and demonstrates for the first time the 
      ability of SPP to activate the transcription factor NF-kappa B in these cells. 
      Furthermore, calcium release from the internal stores by thapsigargin (TG), an 
      inhibitor of the endoplasmic reticulum Ca2+ pump, was associated with activation 
      of NF-kappa B. Moreover, we have shown that while an intracellular calcium 
      chelator BAPTA/AM was able to inhibit both SPP- and TG-induced NF-kappa B 
      activation, it had no effect on TNF-induced NF-kappa B activation. In addition, 
      SPP-induced NF-kappa B activation was blocked both by cyclosporin A, known to 
      inhibit calcineurin phosphatase activity, and by the antioxidant butylated 
      hydroxyanisole. These observations suggest that intracellular calcium 
      mobilization is required for SPP-induced NF-kappa B activation, which may involve 
      calcineurin- and redox-dependent mechanisms.
FAU - Shatrov, V A
AU  - Shatrov VA
AD  - CJF 94-11 INSERM Cytokines et Immunite Antitumorale, Institut Gustave-Roussy, 
      Villejuif, France.
FAU - Lehmann, V
AU  - Lehmann V
FAU - Chouaib, S
AU  - Chouaib S
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Antioxidants)
RN  - 0 (Chelating Agents)
RN  - 0 (DNA Probes)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Indoles)
RN  - 0 (Lysophospholipids)
RN  - 0 (NF-kappa B)
RN  - 0 (Sphingolipids)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 25013-16-5 (Butylated Hydroxyanisole)
RN  - 26993-30-6 (sphingosine 1-phosphate)
RN  - 526U7A2651 (Egtazic Acid)
RN  - 67526-95-8 (Thapsigargin)
RN  - 83HN0GTJ6D (Cyclosporine)
RN  - EC 1.13.12.- (Luciferases)
RN  - K22DDW77C0 (1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid)
RN  - N18RMK75W1 (indo-1)
RN  - NGZ37HRE42 (Sphingosine)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Antioxidants/pharmacology
MH  - Butylated Hydroxyanisole/pharmacology
MH  - Calcium/*metabolism
MH  - Chelating Agents/pharmacology
MH  - Cyclosporine/pharmacology
MH  - DNA Probes
MH  - Egtazic Acid/analogs & derivatives/pharmacology
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Enzyme Inhibitors/pharmacology
MH  - Fluorescent Dyes/metabolism
MH  - Genes, Reporter
MH  - Humans
MH  - Indoles/metabolism
MH  - Leukemia, Myeloid
MH  - Luciferases/metabolism
MH  - *Lysophospholipids
MH  - NF-kappa B/*metabolism
MH  - Sphingolipids/metabolism
MH  - Sphingosine/*analogs & derivatives/pharmacology
MH  - Thapsigargin/pharmacology
MH  - Tumor Cells, Cultured
MH  - Tumor Necrosis Factor-alpha/pharmacology
EDAT- 1997/05/08 00:00
MHDA- 1997/05/08 00:01
CRDT- 1997/05/08 00:00
PHST- 1997/05/08 00:00 [pubmed]
PHST- 1997/05/08 00:01 [medline]
PHST- 1997/05/08 00:00 [entrez]
AID - S0006-291X(97)96598-0 [pii]
AID - 10.1006/bbrc.1997.6598 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 1997 May 8;234(1):121-4. doi: 10.1006/bbrc.1997.6598.