PMID- 9169028
OWN - NLM
STAT- MEDLINE
DCOM- 19970618
LR  - 20190816
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 95
IP  - 2
DP  - 1997 Jun
TI  - Genetic aberrations in pediatric acute lymphoblastic leukemia by comparative 
      genomic hybridization.
PG  - 123-9
AB  - Classical cytogenetic analysis plays an important role in the diagnosis and 
      classification of childhood acute lymphoblastic leukemia (ALL). However, poor in 
      vitro growth of the malignant cells and suboptimal quality of metaphase spreads 
      may sometimes cause false-negative findings (normal karyotype). We used 
      comparative genomic hybridization (CGH) to study whether this new method is able 
      to detect and characterize genetic aberrations not detected by karyotyping. CGH 
      showed clonal genetic aberrations in 8 of 13 cases, most of which showed gains of 
      several chromosomes, indicating hyperdiploidy. The sensitivity of CGH was 
      sufficient to detect a small interstitial deletion of 6q. One karyotypically 
      complex case was resolved by CGH showing a high-level amplification of DNA 
      sequences originating from the 12p12-13. Interphase fluorescence in situ 
      hybridization (FISH) analyses confirmed the CGH findings in 2 cases, validating 
      the accuracy of CGH. In conclusion, CGH experiments established the known fact 
      that hyperdiploidy is the most common finding in pediatric ALLs and that CGH may 
      detect aberrations that are not seen in the G-banded karyotype. CGH was also able 
      to further characterize genetic aberrations such as gene amplification, which is 
      occasionally involved in pediatric ALL as well as in other leukemias.
FAU - Karhu, R
AU  - Karhu R
AD  - Laboratory of Cancer Genetics, Tampere University Hospital, Finland.
FAU - Siitonen, S
AU  - Siitonen S
FAU - Tanner, M
AU  - Tanner M
FAU - Keinanen, M
AU  - Keinanen M
FAU - Makipernaa, A
AU  - Makipernaa A
FAU - Lehtinen, M
AU  - Lehtinen M
FAU - Vilpo, J A
AU  - Vilpo JA
FAU - Isola, J
AU  - Isola J
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
SB  - IM
MH  - Child
MH  - *Chromosome Aberrations
MH  - Female
MH  - Flow Cytometry
MH  - Humans
MH  - Karyotyping
MH  - Male
MH  - Nucleic Acid Hybridization/*methods
MH  - Precursor Cell Lymphoblastic Leukemia-Lymphoma/*genetics
EDAT- 1997/06/01 00:00
MHDA- 1997/06/01 00:01
CRDT- 1997/06/01 00:00
PHST- 1997/06/01 00:00 [pubmed]
PHST- 1997/06/01 00:01 [medline]
PHST- 1997/06/01 00:00 [entrez]
AID - S0165460896002427 [pii]
AID - 10.1016/s0165-4608(96)00242-7 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 1997 Jun;95(2):123-9. doi: 10.1016/s0165-4608(96)00242-7.