PMID- 9169029
OWN - NLM
STAT- MEDLINE
DCOM- 19970618
LR  - 20190816
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 95
IP  - 2
DP  - 1997 Jun
TI  - Telomeric fusions in cultured human fibroblasts as a source of genomic 
      instability.
PG  - 130-6
AB  - In a human fibroblast clone we studied the evolution, during culture propagation, 
      of a dicentric chromosome consisting of the end-to-end association of the short 
      arm of chromosome 5 and the long arm of chromosome 16. Dual-color fluorescence in 
      situ hybridization (FISH) with painting probes allowed us to define the structure 
      of a variety of derivative chromosomes and to identify the mechanisms by which 
      they originated. Asymmetric interchanges involving the intercentromeric region of 
      the dicentric, bridge-breakage-fusion events, or breaks followed by sister 
      chromatid fusion, originate unstable hetero- or homodicentric chromosomes with 
      deletion or duplication; breakages not followed by reunion, or intradicentric 
      recombination, presumably originate stable rearranged monocentric chromosomes. 
      The variety of the derivatives is extremely large because the observed events may 
      involve any site of the intercentromeric region, although the majority of them 
      occurs after a break in 16qh. The results of this investigation document the 
      evolution through successive steps of a telomeric fusion, a chromosome anomaly 
      frequently observed in tumor and senescent cells. They also demonstrate that in 
      cultured cells of normal origin, starting with this anomaly, various chromosomal 
      mechanisms may produce translocations, duplications, and deletions. The karyotype 
      instability produced by a telomeric fusion can be relevant for carcinogenesis 
      because it may generate genetic changes critical in the multistep process of 
      transformation.
FAU - Riboni, R
AU  - Riboni R
AD  - Istituto di Genetica Biochimica ed Evoluzionistica, Consiglio Nazionale delle 
      Ricerche, Pavia, Italy.
FAU - Casati, A
AU  - Casati A
FAU - Nardo, T
AU  - Nardo T
FAU - Zaccaro, E
AU  - Zaccaro E
FAU - Ferretti, L
AU  - Ferretti L
FAU - Nuzzo, F
AU  - Nuzzo F
FAU - Mondello, C
AU  - Mondello C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
SB  - IM
MH  - Cells, Cultured
MH  - *Chromosome Aberrations
MH  - Fibroblasts/*ultrastructure
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - *Telomere
EDAT- 1997/06/01 00:00
MHDA- 1997/06/01 00:01
CRDT- 1997/06/01 00:00
PHST- 1997/06/01 00:00 [pubmed]
PHST- 1997/06/01 00:01 [medline]
PHST- 1997/06/01 00:00 [entrez]
AID - S0165460896002488 [pii]
AID - 10.1016/s0165-4608(96)00248-8 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 1997 Jun;95(2):130-6. doi: 10.1016/s0165-4608(96)00248-8.