PMID- 9169041
OWN - NLM
STAT- MEDLINE
DCOM- 19970618
LR  - 20190816
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 95
IP  - 2
DP  - 1997 Jun
TI  - Molecular characterization of a complex chromosomal rearrangement in a 
      pleomorphic salivary gland adenoma involving the 3'-UTR of HMGIC.
PG  - 198-205
AB  - Recently, the high mobility group protein gene, HMGIC, was identified as a common 
      genetic denominator in benign tumors with chromosome 12q13-15 aberrations, such 
      as lipomas, uterine leiomyomas, pleomorphic adenoma of the salivary glands, 
      hamartomas of breast and lung, angiomyxomas, and endometrial polyps. In most 
      cases, the rearrangements resulted in the separation of the three HMGIC 
      DNA-binding motifs from the acidic carboxy-terminal tail. Here, we report about 
      the molecular characterization of a case of pleomorphic adenoma carrying a 
      t(1;12)(p22;q15). Studies were performed on a cell line derived from the primary 
      tumor, i.e., cell line Ad-312/SV40. Although the chromosome 12 breakpoint was 
      initially mapped more than 1 Mb distal to the HMGIC gene by fluorescence in situ 
      hybridization (FISH) analysis, the present molecular studies reveal a more 
      complex chromosomal rearrangement that directly affects the HMGIC gene. Using 
      3'-RACE analysis, a HMGIC fusion transcript was detected that contained the 
      complete HMGIC, coding region but lacked the putative mRNA destabilizing AUUUA 
      motifs that are normally present in the 3'-UTR of HMGIC. Wild-type HMGIC 
      transcripts were also detected in the tumor cells. The results suggest that 
      alterations in the 3'-noncoding region of HMGIC may have to be considered as 
      pathogenetically relevant.
FAU - Geurts, J M
AU  - Geurts JM
AD  - Laboratory for Molecular Oncology, University of Leuven, Belgium.
FAU - Schoenmakers, E F
AU  - Schoenmakers EF
FAU - Van de Ven, W J
AU  - Van de Ven WJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
RN  - 0 (DNA, Neoplasm)
RN  - 0 (High Mobility Group Proteins)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Adenoma/*genetics
MH  - Base Sequence
MH  - Blotting, Southern
MH  - Chromosomes, Artificial, Yeast
MH  - Chromosomes, Human, Pair 1
MH  - Chromosomes, Human, Pair 12
MH  - Cosmids
MH  - DNA, Neoplasm
MH  - High Mobility Group Proteins/*genetics
MH  - Humans
MH  - Molecular Sequence Data
MH  - RNA, Messenger/genetics
MH  - Salivary Gland Neoplasms/*genetics
MH  - Translocation, Genetic
EDAT- 1997/06/01 00:00
MHDA- 1997/06/01 00:01
CRDT- 1997/06/01 00:00
PHST- 1997/06/01 00:00 [pubmed]
PHST- 1997/06/01 00:01 [medline]
PHST- 1997/06/01 00:00 [entrez]
AID - S0165460896004116 [pii]
AID - 10.1016/s0165-4608(96)00411-6 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 1997 Jun;95(2):198-205. doi: 
      10.1016/s0165-4608(96)00411-6.