PMID- 9169480
OWN - NLM
STAT- MEDLINE
DCOM- 19970626
LR  - 20220311
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 272
IP  - 23
DP  - 1997 Jun 6
TI  - The T cell-directed CC chemokine TARC is a highly specific biological ligand for 
      CC chemokine receptor 4.
PG  - 15036-42
AB  - Thymus and activation-regulated chemokine (TARC) is a recently identified CC 
      chemokine that is expressed constitutively in thymus and transiently in 
      stimulated peripheral blood mononuclear cells. TARC functions as a selective 
      chemoattractant for T cells that express a class of receptors binding TARC with 
      high affinity and specificity. To identify the receptor for TARC, we produced 
      TARC as a fusion protein with secreted alkaline phosphatase (SEAP) and used it 
      for specific binding. By stably transfecting five orphan receptors and five known 
      CC chemokine receptors (CCR1 to -5) into K562 cells, we found that TARC-SEAP 
      bound selectively to cells expressing CCR4. TARC-SEAP also bound to K562 cells 
      stably expressing CCR4 with a high affinity (Kd = 0.5 nM). Only TARC and not five 
      other CC chemokines (MCP-1 (monocyte chemoattractant protein-1), RANTES 
      (regulated upon activation, normal T cells expressed and secreted), MIP-1alpha 
      (macrophage inflammatory protein-1alpha), MIP-1beta, and LARC (liver and 
      activation-regulated chemokine)) competed with TARC-SEAP for binding to CCR4. 
      TARC but not RANTES or MIP-1alpha induced migration and calcium mobilization in 
      293/EBNA-1 cells stably expressing CCR4. K562 cells stably expressing CCR4 also 
      responded to TARC in a calcium mobilization assay. Northern blot analysis 
      revealed that CCR4 mRNA was expressed strongly in human T cell lines and 
      peripheral blood T cells but not in B cells, natural killer cells, monocytes, or 
      granulocytes. Taken together, TARC is a specific functional ligand for CCR4, and 
      CCR4 is the specific receptor for TARC selectively expressed on T cells.
FAU - Imai, T
AU  - Imai T
AD  - Shionogi Institute for Medical Science, 2-5-1 Mishima, Settsu-shi, Osaka 566, 
      Japan. toshio.imai@shionogi.co.jp
FAU - Baba, M
AU  - Baba M
FAU - Nishimura, M
AU  - Nishimura M
FAU - Kakizaki, M
AU  - Kakizaki M
FAU - Takagi, S
AU  - Takagi S
FAU - Yoshie, O
AU  - Yoshie O
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (CCL17 protein, human)
RN  - 0 (CCR4 protein, human)
RN  - 0 (Chemokine CCL17)
RN  - 0 (Chemokine CCL5)
RN  - 0 (Chemokines)
RN  - 0 (Chemokines, CC)
RN  - 0 (Receptors, CCR4)
RN  - 0 (Receptors, Chemokine)
RN  - 0 (Receptors, Cytokine)
RN  - 0 (Recombinant Fusion Proteins)
RN  - EC 3.1.3.1 (Alkaline Phosphatase)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Alkaline Phosphatase/biosynthesis
MH  - B-Lymphocytes/immunology
MH  - Binding, Competitive
MH  - Calcium/metabolism
MH  - Cell Line
MH  - Chemokine CCL17
MH  - Chemokine CCL5/pharmacology
MH  - Chemokines/*metabolism/*pharmacology
MH  - *Chemokines, CC
MH  - Chemotaxis
MH  - Humans
MH  - Kinetics
MH  - Polymerase Chain Reaction
MH  - Receptors, CCR4
MH  - *Receptors, Chemokine
MH  - Receptors, Cytokine/biosynthesis/*metabolism
MH  - Recombinant Fusion Proteins/biosynthesis/metabolism
MH  - T-Lymphocytes/*immunology
MH  - Transfection
MH  - Tumor Cells, Cultured
EDAT- 1997/06/06 00:00
MHDA- 1997/06/06 00:01
CRDT- 1997/06/06 00:00
PHST- 1997/06/06 00:00 [pubmed]
PHST- 1997/06/06 00:01 [medline]
PHST- 1997/06/06 00:00 [entrez]
AID - S0021-9258(19)62495-6 [pii]
AID - 10.1074/jbc.272.23.15036 [doi]
PST - ppublish
SO  - J Biol Chem. 1997 Jun 6;272(23):15036-42. doi: 10.1074/jbc.272.23.15036.