PMID- 9170243
OWN - NLM
STAT- MEDLINE
DCOM- 19970716
LR  - 20190909
IS  - 0273-2289 (Print)
IS  - 0273-2289 (Linking)
VI  - 63-65
DP  - 1997 Spring
TI  - Regulation of phosphotransferases in glucose- and xylose-fermenting yeasts.
PG  - 97-108
AB  - This research examined the titers of hexokinase (HK), phosphofructokinase (PFK), 
      and xylulokinase (XUK) in Saccharomyces cerevisiae and two xylose fermenting 
      yeasts, Pachysolen tannophilus and Candida shehatae, following shifts in carbon 
      source and aeration. Xylose-grown C. shehatae, glucose-grown P. tannophilus, and 
      glucose-grown S. cerevisiae, had the highest specific activities of XUK, HK, and 
      PFK, respectively. XUK was induced by xylose to moderate levels in both P. 
      tannophilus and C. shehatae, but was present only in trace levels in S. 
      cerevisiae. HK activities in P. tannophilus were two to three fold higher when 
      cells were grown on glucose than when grown on xylose, but HK levels were less 
      inducible in C. shehatae. The PFK activities in S. cerevisiae were 1.5 to 2 times 
      higher than in the two xylose-fermenting yeasts. Transfer from glucose to xylose 
      rapidly inactivated HK in P. tannophilus, and transfer from xylose to glucose 
      inactivated XUK in C. shehatae. The patterns of induction and inactivation 
      indicate that the basic regulatory mechanisms differ in the two xylose fermenting 
      yeasts.
FAU - Yang, V W
AU  - Yang VW
AD  - Institute for Microbial and Biochemical Technology, Forest Products Laboratory, 
      Madison, WI 53705-2398, USA.
FAU - Jeffries, T W
AU  - Jeffries TW
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - United States
TA  - Appl Biochem Biotechnol
JT  - Applied biochemistry and biotechnology
JID - 8208561
RN  - A1TA934AKO (Xylose)
RN  - EC 2.7.- (Phosphotransferases)
RN  - EC 2.7.1.- (Phosphotransferases (Alcohol Group Acceptor))
RN  - EC 2.7.1.1 (Hexokinase)
RN  - EC 2.7.1.11 (Phosphofructokinase-1)
RN  - EC 2.7.1.17 (xylulokinase)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - Aerobiosis
MH  - Anaerobiosis
MH  - Candida/metabolism
MH  - Fermentation
MH  - Glucose/*metabolism
MH  - Hexokinase/metabolism
MH  - Kinetics
MH  - Phosphofructokinase-1/metabolism
MH  - Phosphotransferases/*metabolism
MH  - Phosphotransferases (Alcohol Group Acceptor)/metabolism
MH  - Saccharomyces cerevisiae/metabolism
MH  - Saccharomycetales/metabolism
MH  - Species Specificity
MH  - Xylose/*metabolism
MH  - Yeasts/growth & development/*metabolism
EDAT- 1997/04/01 00:00
MHDA- 1997/04/01 00:01
CRDT- 1997/04/01 00:00
PHST- 1997/04/01 00:00 [pubmed]
PHST- 1997/04/01 00:01 [medline]
PHST- 1997/04/01 00:00 [entrez]
AID - 10.1007/BF02920416 [doi]
PST - ppublish
SO  - Appl Biochem Biotechnol. 1997 Spring;63-65:97-108. doi: 10.1007/BF02920416.