PMID- 9170648
OWN - NLM
STAT- MEDLINE
DCOM- 19970702
LR  - 20190729
IS  - 1060-3271 (Print)
IS  - 1060-3271 (Linking)
VI  - 80
IP  - 3
DP  - 1997 May-Jun
TI  - Liquid chromatographic determination of acriflavine and proflavine residues in 
      channel catfish muscle.
PG  - 486-90
AB  - A liquid chromatographic (LC) method was developed for determination of 
      acriflavine (ACR) and proflavine (PRO) residues in channel catfish muscle. 
      Residues were extracted with acidified methanol solution, and extracts were 
      cleaned up with C18 solid-phase extraction columns. Residue concentrations were 
      determined on an LC cyano column, with spectrophotometric detection at 454 nm. 
      Catfish muscle was individually fortified with ACR (purified from commercial 
      product) and PRO at concentrations of 5, 10, 20, 40, and 80 ppb (5 replicates per 
      level). Mean recoveries from fortified muscle at each level ranged from 86 to 
      95%, with relative standard deviations (RSDs) of 2.5 to 5.7%. The method was 
      applied to incurred residues of ACR and PRO in muscle after waterborne exposure 
      of channel catfish to commercial acriflavine (10 ppm total dye for 4 h). RSDs for 
      incurred residues of ACR and PRO were in the same range as those for fortified 
      muscle. Low residue concentrations (< 1% of exposure water concentration) 
      suggested poor absorption of ACR and PRO in catfish.
FAU - Plakas, S M
AU  - Plakas SM
AD  - U.S. Food and Drug Administration, Gulf Coast Seafood Laboratory, Dauphin Island, 
      AL 36528, USA.
FAU - el Said, K R
AU  - el Said KR
FAU - Jester, E L
AU  - Jester EL
FAU - Bencsath, F A
AU  - Bencsath FA
FAU - Hayton, W L
AU  - Hayton WL
LA  - eng
PT  - Journal Article
PL  - England
TA  - J AOAC Int
JT  - Journal of AOAC International
JID - 9215446
RN  - 0 (Anti-Infective Agents, Local)
RN  - 0 (Fluorescent Dyes)
RN  - 1T3A50395T (Acriflavine)
RN  - CY3RNB3K4T (Proflavine)
SB  - IM
MH  - Acriflavine/*analysis
MH  - Animals
MH  - Anti-Infective Agents, Local/*analysis
MH  - *Chromatography, Liquid
MH  - Drug Residues/*analysis
MH  - Fluorescent Dyes/*analysis
MH  - Ictaluridae
MH  - Molecular Structure
MH  - Muscles/chemistry
MH  - Proflavine/*analysis
EDAT- 1997/05/01 00:00
MHDA- 1997/05/01 00:01
CRDT- 1997/05/01 00:00
PHST- 1997/05/01 00:00 [pubmed]
PHST- 1997/05/01 00:01 [medline]
PHST- 1997/05/01 00:00 [entrez]
PST - ppublish
SO  - J AOAC Int. 1997 May-Jun;80(3):486-90.