PMID- 9172015
OWN - NLM
STAT- MEDLINE
DCOM- 19970818
LR  - 20190909
IS  - 0192-0561 (Print)
IS  - 0192-0561 (Linking)
VI  - 18
IP  - 12
DP  - 1996 Dec
TI  - (+/-)-3-[4-(2-dimethylamino-1-methylethoxy)-phenyl]-1H-pyrazolo[3,4- 
      B]pyridine-1-acetic acid (Y-25510) stimulates production of IL-1 beta and IL-6 at 
      the level of messenger RNA expression in cultured human monocytes.
PG  - 719-28
AB  - (+/-)-3-[4-(2-Dimethylamino-1-methylethoxy)phenyl]-1H-pyrazolo[3, 
      4-b]pyridine-1-acetic acid (Y-25510) stimulated the mRNA expression for 
      interleukin-1 beta (IL-1 beta), and enhanced the expression induced by 
      lipopolysaccharide (LPS) in cultured human peripheral blood mononuclear cells 
      (PBMC) and THP-1 cells, a cell-line derived from human monocytic leukemia. 
      Y-25510 also stimulated the mRNA expression for IL-6 in both types of the cells, 
      however, the stimulation required the presence of LPS. In THP-1 cells, the 
      stimulation of IL-1 beta mRNA expression by Y-25510 was suppressed by 
      cycloheximide, an inhibitor of protein synthesis. This phenomenon indicates that 
      the stimulation requires de norv protein synthesis. In contrast, the stimulation 
      of mRNA expression for IL-6 by Y-25510 was not suppressed by cycloheximide but 
      suppressed by N alpha-p-tosyl-L-phenylalanine chloromethyl ketone (TPCK), an 
      inhibitor of nuclear transcription factor-kappa B (NF-kappa B) activation, in the 
      presence of LPS, suggesting that the stimulation requires NF-kappa activation. 
      These results demonstrate that Y-25510 stimulates the mRNA expression for IL-1 
      beta and IL-6 by different mechanisms. Dexamethasone suppressed the LPS-induced 
      expression of mRNA for IL-1 beta and IL-6 in THP-1 cells, whereas the drug never 
      suppressed the mRNA expression for these cytokines in the presence of Y-25510. 
      The result indicates that Y-25510 stimulates the mRNA expression for IL-1 beta 
      and IL-6 by different mechanisms from those of LPS.
FAU - Kusuhara, H
AU  - Kusuhara H
AD  - Research Laboratories, Yoshitomi Pharmaceutical Industries Ltd, Fukuoka, Japan.
FAU - Komatsu, H
AU  - Komatsu H
FAU - Hisadome, M
AU  - Hisadome M
FAU - Ikeda, Y
AU  - Ikeda Y
LA  - eng
PT  - Journal Article
PL  - England
TA  - Int J Immunopharmacol
JT  - International journal of immunopharmacology
JID - 7904799
RN  - 0 (Culture Media)
RN  - 0 (Interleukin-1)
RN  - 0 (Interleukin-6)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Pyrazoles)
RN  - 0 (Pyridines)
RN  - 0 (RNA, Messenger)
RN  - 145194-32-7 (Y 25510)
RN  - 402-71-1 (Tosylphenylalanyl Chloromethyl Ketone)
RN  - 7S5I7G3JQL (Dexamethasone)
RN  - 98600C0908 (Cycloheximide)
SB  - IM
MH  - Culture Media/metabolism/pharmacology
MH  - Cycloheximide/pharmacology
MH  - Dexamethasone/metabolism/pharmacology
MH  - Humans
MH  - Interleukin-1/*biosynthesis/genetics
MH  - Interleukin-6/*biosynthesis/genetics
MH  - Lipopolysaccharides/pharmacology
MH  - Monocytes/*metabolism
MH  - Pyrazoles/*pharmacology
MH  - Pyridines/*pharmacology
MH  - RNA, Messenger/*metabolism
MH  - Tosylphenylalanyl Chloromethyl Ketone/pharmacology
MH  - Tumor Cells, Cultured
EDAT- 1996/12/01 00:00
MHDA- 1996/12/01 00:01
CRDT- 1996/12/01 00:00
PHST- 1996/12/01 00:00 [pubmed]
PHST- 1996/12/01 00:01 [medline]
PHST- 1996/12/01 00:00 [entrez]
AID - S0192056197855543 [pii]
AID - 10.1016/s0192-0561(97)85554-3 [doi]
PST - ppublish
SO  - Int J Immunopharmacol. 1996 Dec;18(12):719-28. doi: 
      10.1016/s0192-0561(97)85554-3.