PMID- 9172017
OWN - NLM
STAT- MEDLINE
DCOM- 19970818
LR  - 20190909
IS  - 0192-0561 (Print)
IS  - 0192-0561 (Linking)
VI  - 18
IP  - 12
DP  - 1996 Dec
TI  - Differential regulation of TNF alpha, IL-1 beta, IL-6, IL-8, TNF beta, and IL-10 
      by pentoxifylline.
PG  - 739-48
AB  - Pentoxifylline (PTX) is a methylxanthine drug known to inhibit the production of 
      tumor necrosis factor-alpha (TNF alpha), which plays a key role in inflammation. 
      Recent studies also revealed that other cytokines may be inhibited by PTX. We 
      investigated PTX effects on production and mRNA expression of TNF alpha, IL-1 
      beta, IL-6, IL-8, TNF beta and IL-10. Cytokine release was studied in 1/10 
      diluted whole blood culture (WB) and in peripheral blood mononuclear cell (PBMC) 
      culture. Cytokine production was triggered in both culture systems by endotoxin 
      (LPS) or by phorbol ester (PMA) plus phytohemagglutinin (PHA). Our results showed 
      that expression and production of TNF alpha and TNF beta were inhibited by PTX in 
      a dose-dependent manner. Moreover, we observed that depending on the way of 
      activating cells, PTX induced an up- or a down-regulation (in PMA + PHA or LPS 
      stimulated cells, respectively) for IL-1 and IL-6 release. We also noted that the 
      effects of PTX on IL-6, IL-8 and IL-10 production were different in WB and in 
      PBMC culture. In conclusion PTX acts on cytokine in a complex manner depending on 
      cellular environment and on the method of activation.
FAU - D'Hellencourt, C L
AU  - D'Hellencourt CL
AD  - Laboratoire d'Hematologie, C.H.R. Robert Debre, Reims, France. 
      Hellenco@angers.inserm.fr
FAU - Diaw, L
AU  - Diaw L
FAU - Cornillet, P
AU  - Cornillet P
FAU - Guenounou, M
AU  - Guenounou M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Int J Immunopharmacol
JT  - International journal of immunopharmacology
JID - 7904799
RN  - 0 (Anti-Inflammatory Agents)
RN  - 0 (Cytokines)
RN  - 0 (Interleukin-1)
RN  - 0 (Interleukin-6)
RN  - 0 (Interleukin-8)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Lymphotoxin-alpha)
RN  - 0 (Phytohemagglutinins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (Vasodilator Agents)
RN  - 130068-27-8 (Interleukin-10)
RN  - NI40JAQ945 (Tetradecanoylphorbol Acetate)
RN  - SD6QCT3TSU (Pentoxifylline)
SB  - IM
MH  - Anti-Inflammatory Agents/pharmacology
MH  - Cytokines/biosynthesis/drug effects/genetics
MH  - Down-Regulation/drug effects
MH  - Gene Expression
MH  - Gene Expression Regulation
MH  - Humans
MH  - Interleukin-1/*antagonists & inhibitors/*genetics
MH  - Interleukin-10/*antagonists & inhibitors/*genetics
MH  - Interleukin-6/*antagonists & inhibitors/*genetics
MH  - Interleukin-8/*antagonists & inhibitors/*genetics
MH  - Leukocytes, Mononuclear/metabolism
MH  - Lipopolysaccharides/pharmacology
MH  - Lymphotoxin-alpha/*antagonists & inhibitors/*genetics
MH  - Pentoxifylline/*pharmacology
MH  - Phytohemagglutinins/pharmacology
MH  - RNA, Messenger/metabolism
MH  - Tetradecanoylphorbol Acetate/pharmacology
MH  - Tumor Necrosis Factor-alpha/*antagonists & inhibitors/*genetics
MH  - Vasodilator Agents/*pharmacology
EDAT- 1996/12/01 00:00
MHDA- 1996/12/01 00:01
CRDT- 1996/12/01 00:00
PHST- 1996/12/01 00:00 [pubmed]
PHST- 1996/12/01 00:01 [medline]
PHST- 1996/12/01 00:00 [entrez]
AID - S0192056197855567 [pii]
AID - 10.1016/s0192-0561(97)85556-7 [doi]
PST - ppublish
SO  - Int J Immunopharmacol. 1996 Dec;18(12):739-48. doi: 
      10.1016/s0192-0561(97)85556-7.