PMID- 9174122
OWN - NLM
STAT- MEDLINE
DCOM- 19970619
LR  - 20191024
IS  - 0262-0898 (Print)
IS  - 0262-0898 (Linking)
VI  - 15
IP  - 3
DP  - 1997 May
TI  - Glucocorticoid receptor function possibly modulates cell-cell interactions in 
      osteoblastic metastases on rat skeleton.
PG  - 205-17
AB  - We analysed the glucocorticoid receptor (GR) function and its ability to modulate 
      cell-cell interactions between the PA-III rat prostate cancer and UMR 106 
      osteoblast-like rat osteosarcoma cells as an in vitro model for studying GR 
      function in PA-III cell-induced tumor and blastic reaction in rat bone. Intact GR 
      was detected by ligand binding assays, DNA band-shift, and GR trans-activation 
      analysis of PA-III and UMR 106 cells transiently transfected with the mouse 
      mammary tumor virus thymidine kinase-chloramphenicol acetyltransferase reporter 
      gene. Dexamethasone and transforming growth factor beta 1 (TGFbeta1) inhibited 
      the growth of PA-III and UMR 106 cells. Dexamethasone's inhibition of PA-III and 
      UMR 106 cells was reversed by anti-TGFbeta1 antibody and exogenous insulin-like 
      growth factor I (IGF-I). Exogenous IGF-I, urokinase-type plasminogen activator 
      (uPA), UMR 106 conditioned media (CM) and PA-III CM stimulated the proliferation 
      of PA-III and UMR 106 cells. The mitogenic activity exerted by uPA and PA-III CM 
      in UMR 106 cells was completely neutralized by anti-IGF-I specific antibody. In 
      addition, dexamethasone up-regulated TGFbeta1 mRNA and down-regulated uPA mRNA 
      expression in PA-III cells without affecting TGFbeta1 and uPA mRNA expression in 
      UMR 106 cells. These data suggested that TGFbeta1, uPA, and IGF-I mediate at 
      least in part cell-cell interactions and GR function in PA-III prostate cancer 
      and UMR 106 osteosarcoma cells.
FAU - Reyes-Moreno, C
AU  - Reyes-Moreno C
AD  - Molecular Endocrinology Laboratory, Research Center, Centre Hospitalier de 
      L'Universite Laval (C.H.U.L.), St Foy, Quebec, Canada.
FAU - Koutsilieris, M
AU  - Koutsilieris M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Clin Exp Metastasis
JT  - Clinical & experimental metastasis
JID - 8409970
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Glucocorticoid)
RN  - 0 (Transforming Growth Factor beta)
RN  - 67763-96-6 (Insulin-Like Growth Factor I)
RN  - 7S5I7G3JQL (Dexamethasone)
RN  - 9007-49-2 (DNA)
RN  - EC 3.4.21.73 (Urokinase-Type Plasminogen Activator)
SB  - IM
MH  - Animals
MH  - Bone Neoplasms/pathology/*secondary
MH  - *Cell Communication
MH  - Cell Division
MH  - DNA/metabolism
MH  - Dexamethasone/pharmacology
MH  - Humans
MH  - Insulin-Like Growth Factor I/pharmacology
MH  - Male
MH  - Osteosarcoma/*pathology/physiopathology
MH  - Prostatic Neoplasms/*pathology/physiopathology
MH  - RNA, Messenger/analysis
MH  - Rats
MH  - Receptors, Glucocorticoid/*physiology
MH  - Transcriptional Activation
MH  - Transforming Growth Factor beta/genetics/pharmacology
MH  - Tumor Cells, Cultured
MH  - Urokinase-Type Plasminogen Activator/genetics/pharmacology
EDAT- 1997/05/01 00:00
MHDA- 1997/05/01 00:01
CRDT- 1997/05/01 00:00
PHST- 1997/05/01 00:00 [pubmed]
PHST- 1997/05/01 00:01 [medline]
PHST- 1997/05/01 00:00 [entrez]
AID - 10.1023/a:1018413229570 [doi]
PST - ppublish
SO  - Clin Exp Metastasis. 1997 May;15(3):205-17. doi: 10.1023/a:1018413229570.