PMID- 9174628
OWN - NLM
STAT- MEDLINE
DCOM- 19970623
LR  - 20190920
IS  - 0945-6317 (Print)
IS  - 0945-6317 (Linking)
VI  - 430
IP  - 5
DP  - 1997 May
TI  - Detection of karyotype changes in interphase cells: oligonucleotide-primed in 
      situ labelling versus fluorescence in situ hybridization.
PG  - 381-7
AB  - Interphase cytogenetics is a rapidly developing technique which is usually 
      performed by fluorescence in situ hybridization (FISH). Recently, 
      oligonucleotide-primed in situ synthesis (PRINS) has become established as a 
      method of labelling centromeric regions of chromosomes in metaphase spreads. We 
      tested the suitability of PRINS in detecting the exact copy number of chromosomes 
      1, 3, 7 and 8 in intact interphase cells of 17 cytological preparations derived 
      from normal and neoplastic tissues. Control procedures consisted in preparation 
      of metaphase spreads of lymphocytes of healthy donors, conventional cytogenetics 
      in some of the specimens, and omission of the primers or Taq polymerase from the 
      reaction mixture. All specimens were additionally examined by FISH and analysed 
      blind by two experienced observers. Both PRINS and FISH revealed a corresponding 
      distribution of hybridization signals for all chromosomes examined in specimens 
      of normal bone marrow (n = 5), normal liver cells (n = 5), three samples of acute 
      nonlymphocytic leukaemia in which conventional chromosome analyses had shown 
      monosomy 7 or trisomy 8, and in four hepatocellular carcinomas that displayed 
      trisomy 1. Overall, statistical analysis revealed no significant difference in 
      the signal distribution between the two techniques. Our results demonstrate that 
      PRINS is as reliable as FISH for detecting chromosome copy numbers in interphase 
      nuclei of intact cells. The PRINS method, however, is easier to perform, faster 
      and less expensive, holding great potential for future applications in diagnostic 
      pathology.
FAU - Werner, M
AU  - Werner M
AD  - Institute of Pathology, Technical University of Munich, Germany.
FAU - Wilkens, L
AU  - Wilkens L
FAU - Nasarek, A
AU  - Nasarek A
FAU - Tchinda, J
AU  - Tchinda J
FAU - Komminoth, P
AU  - Komminoth P
LA  - eng
PT  - Journal Article
PL  - Germany
TA  - Virchows Arch
JT  - Virchows Archiv : an international journal of pathology
JID - 9423843
RN  - 0 (DNA Primers)
SB  - IM
MH  - Biopsy
MH  - Bone Marrow/*pathology
MH  - Cell Nucleus/ultrastructure
MH  - Chromosomes/ultrastructure
MH  - DNA Primers/*genetics
MH  - Humans
MH  - In Situ Hybridization/*methods
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - *Interphase
MH  - *Karyotyping
MH  - Leukemia, Myeloid, Acute/genetics/pathology
MH  - Liver/pathology
MH  - Lymphocytes/pathology
MH  - Trisomy
EDAT- 1997/05/01 00:00
MHDA- 1997/05/01 00:01
CRDT- 1997/05/01 00:00
PHST- 1997/05/01 00:00 [pubmed]
PHST- 1997/05/01 00:01 [medline]
PHST- 1997/05/01 00:00 [entrez]
AID - 10.1007/s004280050047 [doi]
PST - ppublish
SO  - Virchows Arch. 1997 May;430(5):381-7. doi: 10.1007/s004280050047.