PMID- 9175597
OWN - NLM
STAT- MEDLINE
DCOM- 19970722
LR  - 20190701
IS  - 0304-3940 (Print)
IS  - 0304-3940 (Linking)
VI  - 226
IP  - 3
DP  - 1997 May 2
TI  - Migration of granule neurons in cerebellar organotypic cultures is impaired by 
      methylmercury.
PG  - 183-6
AB  - To examine whether abnormal migration of granule cells in the external granular 
      layer during cerebellar development is in part of the etiology of fetal Minamata 
      disease, organotypic culture of rat cerebellar slice was established. Migration 
      of external granule cells pulse-labeled with bromodeoxyuridine (BrdU) toward the 
      internal granular layer was inhibited by the presence of methylmercury (0-10 
      microM) in a dose-dependent manner. Nuclear condensation and DNA fragmentation 
      visualized by the indirect immunofluorescence method with anti-BrdU antibody and 
      the terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) 
      method indicated that a large fraction of cells in the external granular layer 
      underwent apoptotic death in the slices treated with 10 microM methylmercury. 
      Thus, methylmercury inhibits the migration of cerebellar granule cells in a model 
      system for neural development. The impaired migration was a possible cause of the 
      apoptotic death of external granule cells.
FAU - Kunimoto, M
AU  - Kunimoto M
AD  - Environmental Health Sciences Division, National Institute for Environmental 
      Studies, Tsukuba, Ibaraki, Japan. kunimoto@nies.go.jp
FAU - Suzuki, T
AU  - Suzuki T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Ireland
TA  - Neurosci Lett
JT  - Neuroscience letters
JID - 7600130
RN  - 0 (Deoxyuracil Nucleotides)
RN  - 0 (Methylmercury Compounds)
RN  - EC 2.7.7.31 (DNA Nucleotidylexotransferase)
RN  - G34N38R2N1 (Bromodeoxyuridine)
SB  - IM
MH  - Animals
MH  - Apoptosis
MH  - Bromodeoxyuridine
MH  - Cell Movement/physiology
MH  - Cerebellum/cytology/*drug effects
MH  - DNA Fragmentation/drug effects
MH  - DNA Nucleotidylexotransferase
MH  - Deoxyuracil Nucleotides
MH  - In Situ Hybridization
MH  - Methylmercury Compounds/*pharmacology
MH  - Neurons/cytology/*drug effects
MH  - Organ Culture Techniques
MH  - Rats
MH  - Rats, Wistar
EDAT- 1997/05/02 00:00
MHDA- 1997/05/02 00:01
CRDT- 1997/05/02 00:00
PHST- 1997/05/02 00:00 [pubmed]
PHST- 1997/05/02 00:01 [medline]
PHST- 1997/05/02 00:00 [entrez]
AID - S0304-3940(97)00273-5 [pii]
AID - 10.1016/s0304-3940(97)00273-5 [doi]
PST - ppublish
SO  - Neurosci Lett. 1997 May 2;226(3):183-6. doi: 10.1016/s0304-3940(97)00273-5.