PMID- 9175636
OWN - NLM
STAT- MEDLINE
DCOM- 19970811
LR  - 20190512
IS  - 0267-8357 (Print)
IS  - 0267-8357 (Linking)
VI  - 12
IP  - 3
DP  - 1997 May
TI  - Induction and persistence of cytogenetic damage in mouse splenocytes following 
      whole-body X-irradiation analysed by fluorescence in situ hybridization. III. 
      Chromosome malsegregation/aneuploidy.
PG  - 125-31
AB  - Transgenic mice with foreign DNA inserted into three pairs of chromosomes were 
      exposed to 2 Gy X-rays in order to study the induction and persistence of 
      chromosome malsegregation and aneuploidy up to 28 days after exposure. By tracing 
      the marker chromosomes in cytokinesis-blocked binucleated splenocytes using 
      fluorescence in situ hybridization (FISH), reciprocal products of chromosome 
      malsegregation in the daughter nuclei were analysed. FISH with murine minor 
      satellite DNA was employed to detect chromosome loss (MN with a centromere) in 
      binucleated splenocytes. In addition to its clastogenic effects, X-irradiation 
      also showed aneugenic activity, which was observed as centromere positive 
      micronuclei (C + MN) and malsegregated marker chromosomes detected by FISH. The 
      initial frequency of micronuclei (MN) analysed by Acridine Orange staining 
      immediately after X-ray exposure was found to be 42.3 per 100 binucleated cells. 
      The MN frequency declined in an exponential manner and at day 14, reached about 
      half the value observed immediately after irradiation and 14% MN were detected at 
      day 28. Of these MN, 25% were centromere positive at day 0 as detected by minor 
      satellite signal after FISH. The percentage C + MN increased further at day 3 and 
      declined at day 14 to the level observed at day 0. There were 7.6% malsegregated 
      cells immediately after X-irradiation as analysed by two colour FISH. This value 
      increased further during later intervals and remained stable until day 28. A 
      combination of the Acridine Orange staining and FISH with minor satellite DNA and 
      marker DNA to detect aneuploidy and chromosome malsegregation, was utilized in 
      the present study to demonstrate the induction and persistence of aneugenic and 
      clastogenic damage in transgenic mice irradiated in vivo.
FAU - Hande, M P
AU  - Hande MP
AD  - Department of Radiation Genetics and Chemical Mutagenesis, Leiden University, The 
      Netherlands.
FAU - Boei, J J
AU  - Boei JJ
FAU - Natarajan, A T
AU  - Natarajan AT
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Mutagenesis
JT  - Mutagenesis
JID - 8707812
RN  - 0 (DNA Probes)
RN  - 0 (DNA, Satellite)
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Genetic Markers)
RN  - F30N4O6XVV (Acridine Orange)
SB  - IM
MH  - Acridine Orange
MH  - *Aneuploidy
MH  - Animals
MH  - Bacteriophage lambda/genetics
MH  - *Chromosome Aberrations
MH  - Cytogenetics
MH  - DNA Probes
MH  - DNA, Satellite/genetics
MH  - Female
MH  - Fluorescent Dyes
MH  - Genes, myc
MH  - Genetic Markers
MH  - In Situ Hybridization, Fluorescence
MH  - Mice
MH  - Mice, Transgenic
MH  - Micronucleus Tests
MH  - Spleen/*radiation effects/ultrastructure
MH  - Time Factors
EDAT- 1997/05/01 00:00
MHDA- 1997/05/01 00:01
CRDT- 1997/05/01 00:00
PHST- 1997/05/01 00:00 [pubmed]
PHST- 1997/05/01 00:01 [medline]
PHST- 1997/05/01 00:00 [entrez]
AID - 10.1093/mutage/12.3.125 [doi]
PST - ppublish
SO  - Mutagenesis. 1997 May;12(3):125-31. doi: 10.1093/mutage/12.3.125.