PMID- 9176245
OWN - NLM
STAT- MEDLINE
DCOM- 19970708
LR  - 20171213
IS  - 0002-9513 (Print)
IS  - 0002-9513 (Linking)
VI  - 272
IP  - 5 Pt 1
DP  - 1997 May
TI  - Type II pneumocytes release chemoattractant activity for monocytes 
      constitutively.
PG  - L830-7
AB  - In the present investigation, we determined whether A549 cells, a type II 
      pneumocyte cell line, might release mediators that are responsible for monocyte 
      chemoattractant activity (MCA) constitutively. To test this hypothesis, A549 cell 
      supernatant fluids were harvested and evaluated for monocyte chemotaxis. A549 
      cell supernatant fluids showed MCA in a time-dependent manner (P < 0.001). 
      Checkerboard analysis of 24- and 72-h supernatant fluids showed that the activity 
      was chemokinetic rather than chemotactic. Partial characterization of 24- and 
      72-h supernatant fluids revealed that the mediator was composed of lipid-soluble 
      activity that was blocked by lipoxygenase inhibitors and trypsin-sensitive 
      activity blocked by cycloheximide. Molecular sieve column chromatography 
      identified four molecular weight peaks. Two of four peaks were blocked by 
      anti-monocyte chemoattractant protein-1 (MCP-1) and anti-transforming growth 
      factor-beta (TGF-beta) polyclonal antibodies. MCP-1 and TGF-beta were detected by 
      enzyme-linked immunosorbent assay. Leukotriene B4 (LTB4) receptor antagonist 
      attenuated the lowest-molecular-weight peak chemotactic response, and the 
      concentration of LTB4 was high enough for chemotactic activity. These findings 
      suggest that type II pneumocytes may modulate the recruitment of monocytes into 
      the alveolar space by releasing MCP-1, TGF-beta, and LTB4 constitutively.
FAU - Koyama, S
AU  - Koyama S
AD  - Shinshu University School of Medicine, First Department of Internal Medicine, 
      Matsumoto, Japan.
FAU - Sato, E
AU  - Sato E
FAU - Nomura, H
AU  - Nomura H
FAU - Kubo, K
AU  - Kubo K
FAU - Nagai, S
AU  - Nagai S
FAU - Izumi, T
AU  - Izumi T
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Am J Physiol
JT  - The American journal of physiology
JID - 0370511
RN  - 0 (Antibodies)
RN  - 0 (Cytokines)
RN  - 0 (Monocyte Chemoattractant Proteins)
RN  - 0 (Platelet Activating Factor)
RN  - 0 (Platelet Membrane Glycoproteins)
RN  - 0 (Receptors, Cell Surface)
RN  - 0 (Receptors, G-Protein-Coupled)
RN  - 0 (Receptors, Leukotriene B4)
RN  - 0 (platelet activating factor receptor)
SB  - IM
MH  - Antibodies/immunology
MH  - Cytokines/immunology/metabolism
MH  - Humans
MH  - Lung/*metabolism/pathology
MH  - Monocyte Chemoattractant Proteins/chemistry/*metabolism
MH  - Osmolar Concentration
MH  - Platelet Activating Factor/metabolism
MH  - Platelet Membrane Glycoproteins/antagonists & inhibitors
MH  - *Receptors, Cell Surface
MH  - *Receptors, G-Protein-Coupled
MH  - Receptors, Leukotriene B4/antagonists & inhibitors
MH  - Tumor Cells, Cultured
EDAT- 1997/05/01 00:00
MHDA- 1997/05/01 00:01
CRDT- 1997/05/01 00:00
PHST- 1997/05/01 00:00 [pubmed]
PHST- 1997/05/01 00:01 [medline]
PHST- 1997/05/01 00:00 [entrez]
AID - 10.1152/ajplung.1997.272.5.L830 [doi]
PST - ppublish
SO  - Am J Physiol. 1997 May;272(5 Pt 1):L830-7. doi: 10.1152/ajplung.1997.272.5.L830.