PMID- 9177292
OWN - NLM
STAT- MEDLINE
DCOM- 19970710
LR  - 20220321
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 234
IP  - 2
DP  - 1997 May 19
TI  - Interleukin-18 activates NF-kappaB in murine T helper type 1 cells.
PG  - 454-7
AB  - Interleukin-18 (IL-18) activated T helper type 1 (Th1) cells, OVA#4, and induced 
      production of interleukin-2 (IL-2) in costimulation with anti-CD3 antibody. Upon 
      stimulation with IL-18, IkappaB disappeared from cytoplasm and subsequently 
      nuclear factor-kappaB (NF-kappaB) (p65) accumulated in the nucleus. Corresponding 
      with that, DNA binding activity of NF-kappaB (p65 homodimer or p65/p50 
      heterodimer) was detected in the nucleus. In the transfection experiments, an 
      IL-2 promoter-driven reporter construct showed the similar responsiveness against 
      IL-18 to that of the intrinsic IL-2 gene, and a construct lacking kappaB site 
      failed to respond to IL-18. These results suggest that IL-18 activates NF-kappaB 
      and it is important for enhancement of IL-2 gene expression by Th1 cells 
      stimulated with IL-18.
FAU - Matsumoto, S
AU  - Matsumoto S
AD  - Fujisaki Institute, Hayashibara Biochemical Labs, Inc., Okayama, Japan.
FAU - Tsuji-Takayama, K
AU  - Tsuji-Takayama K
FAU - Aizawa, Y
AU  - Aizawa Y
FAU - Koide, K
AU  - Koide K
FAU - Takeuchi, M
AU  - Takeuchi M
FAU - Ohta, T
AU  - Ohta T
FAU - Kurimoto, M
AU  - Kurimoto M
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Cytokines)
RN  - 0 (DNA Probes)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (I-kappa B Proteins)
RN  - 0 (Interleukin-18)
RN  - 0 (Interleukin-2)
RN  - 0 (NF-kappa B)
RN  - 0 (Nfkbia protein, rat)
RN  - 139874-52-5 (NF-KappaB Inhibitor alpha)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Biological Transport, Active
MH  - Cell Nucleus/metabolism
MH  - Clone Cells
MH  - Cytokines/*pharmacology
MH  - Cytoplasm/metabolism
MH  - DNA/metabolism
MH  - DNA Probes/genetics
MH  - DNA-Binding Proteins/genetics/metabolism
MH  - Genes, Reporter
MH  - *I-kappa B Proteins
MH  - Interleukin-18
MH  - Interleukin-2/biosynthesis/genetics
MH  - Lymphocyte Activation
MH  - NF-KappaB Inhibitor alpha
MH  - NF-kappa B/genetics/*metabolism
MH  - Rats
MH  - Th1 Cells/*immunology/*metabolism
MH  - Transcriptional Activation
MH  - Transfection
EDAT- 1997/05/19 00:00
MHDA- 1997/05/19 00:01
CRDT- 1997/05/19 00:00
PHST- 1997/05/19 00:00 [pubmed]
PHST- 1997/05/19 00:01 [medline]
PHST- 1997/05/19 00:00 [entrez]
AID - S0006-291X(97)96665-1 [pii]
AID - 10.1006/bbrc.1997.6665 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 1997 May 19;234(2):454-7. doi: 
      10.1006/bbrc.1997.6665.