PMID- 9177704
OWN - NLM
STAT- MEDLINE
DCOM- 19970710
LR  - 20071114
IS  - 0003-2697 (Print)
IS  - 0003-2697 (Linking)
VI  - 247
IP  - 2
DP  - 1997 May 1
TI  - Detection of infrequent and multiple K-ras mutations in human tumors and 
      tumor-adjacent tissues.
PG  - 394-403
AB  - A sensitive method was developed and applied to examine the distribution of K-ras 
      gene mutations in histologically differing areas of lung tissues obtained from 
      lung cancer patients. This method, which combines polymerase chain reaction 
      (PCR), mutation allele enrichment (MAE), and denaturing gradient gel 
      electrophoresis (DGGE), allows detection of one K-ras mutant allele present in 
      10(4) to 10(5) wild-type alleles. It was applied to analyze mutations in codon 12 
      of the K-ras gene in 43 tissue sites microdissected from paraffin-embedded 
      sections obtained from 8 archival cases of lung cancer, all previously shown to 
      have codon 12 K-ras mutations by direct sequencing. In four cases, mutations were 
      detected only in the tumor, while in the other four cases, the same mutations 
      were also found in tissues adjacent to tumors, using the MAE + DGGE method. No 
      mutations were detected among normal-appearing cells in areas distant from the 
      tumors in any of the cases studied. These findings demonstrate that K-ras 
      mutations can be detected at low frequencies in normal-appearing cells from 
      tissues adjacent to the tumor in some lung cancer cases. In addition, this 
      approach also allowed detection of multiple mutations in colorectal tissues 
      obtained from colorectal cancer patients. Thus, the MAE + DGGE method may be 
      applicable to study of K-ras mutations in premalignant or morphologically 
      suspicious lesions in bronchial mucosa or other types of human cancer.
FAU - Keohavong, P
AU  - Keohavong P
AD  - Department of Environmental and Occupational Health, University of Pittsburgh, 
      Pennsylvania 15213, USA.
FAU - Zhu, D
AU  - Zhu D
FAU - Whiteside, T L
AU  - Whiteside TL
FAU - Swalsky, P
AU  - Swalsky P
FAU - Bakker, A
AU  - Bakker A
FAU - Elder, E M
AU  - Elder EM
FAU - Siegfried, J M
AU  - Siegfried JM
FAU - Srivastava, S
AU  - Srivastava S
FAU - Finkelstein, S D
AU  - Finkelstein SD
LA  - eng
GR  - 1-RO3-CA71609-01/CA/NCI NIH HHS/United States
GR  - CN-15393-02/CN/NCI NIH HHS/United States
GR  - P20-CA58235/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Anal Biochem
JT  - Analytical biochemistry
JID - 0370535
RN  - 0 (Codon)
RN  - 0 (DNA Primers)
RN  - 0 (DNA, Neoplasm)
SB  - IM
MH  - Base Sequence
MH  - Codon/genetics
MH  - Colorectal Neoplasms/genetics
MH  - DNA Mutational Analysis/*methods/statistics & numerical data
MH  - DNA Primers/genetics
MH  - DNA, Neoplasm/genetics/isolation & purification
MH  - Electrophoresis, Polyacrylamide Gel
MH  - *Genes, ras
MH  - Humans
MH  - Lung Neoplasms/*genetics/pathology
MH  - *Mutation
MH  - Polymerase Chain Reaction/methods/statistics & numerical data
MH  - Sensitivity and Specificity
EDAT- 1997/05/01 00:00
MHDA- 1997/05/01 00:01
CRDT- 1997/05/01 00:00
PHST- 1997/05/01 00:00 [pubmed]
PHST- 1997/05/01 00:01 [medline]
PHST- 1997/05/01 00:00 [entrez]
AID - S0003-2697(97)92100-X [pii]
AID - 10.1006/abio.1997.2100 [doi]
PST - ppublish
SO  - Anal Biochem. 1997 May 1;247(2):394-403. doi: 10.1006/abio.1997.2100.