PMID- 9178771
OWN - NLM
STAT- MEDLINE
DCOM- 19970703
LR  - 20220330
IS  - 0950-9232 (Print)
IS  - 0950-9232 (Linking)
VI  - 14
IP  - 22
DP  - 1997 Jun 5
TI  - Cloning of a gene highly overexpressed in cancer coding for a novel KH-domain 
      containing protein.
PG  - 2729-33
AB  - In a previous large scale screen for differentially expressed genes in pancreatic 
      cancer, we identified a gene highly overexpressed in cancer encoding a novel 
      protein with four K-homologous (KH) domains. KH-domains are found in a subset of 
      RNA-binding proteins, including pre-mRNA-binding (hnRNP) K protein and the 
      fragile X mental retardation gene product (FMR1). By fluorescence in situ 
      hybridization (FISH) the identified gene named koc (KH domain containing protein 
      overexpressed in cancer) was assigned to chromosome 7p11.5. Two pseudogenes were 
      localised on chromosome 6 and 11. The cloned koc cDNA has a 250 bp 5'-UTR, a 1740 
      bp ORF and a 2168 bp 3'-UTR. The AU-rich 3'-untranslated region of koc contains 
      eight AUUUA and four AUUUUUA reiterated motifs. The deduced koc protein with 580 
      amino-acids has a relative molecular mass (Mr) of approximately 65,000 (65 K). 
      The koc transcript is highly overexpressed in pancreatic cancer cell lines and in 
      pancreatic cancer tissue as compared to both, normal pancreas and chronic 
      pancreatitis tissue. High levels of expression were as well found in tissue 
      samples of other human tumours. As the KH domain has been shown to be involved in 
      the regulation of RNA synthesis and metabolism, we speculate that koc may assume 
      a role in the regulation of tumour cell proliferation by interfering with 
      transcriptional and or posttranscriptional processes. However, the precise role 
      of koc in human tumour cells is unknown and remains to be elucidated.
FAU - Mueller-Pillasch, F
AU  - Mueller-Pillasch F
AD  - Department of Internal Medicine I, University of Ulm, Germany.
FAU - Lacher, U
AU  - Lacher U
FAU - Wallrapp, C
AU  - Wallrapp C
FAU - Micha, A
AU  - Micha A
FAU - Zimmerhackl, F
AU  - Zimmerhackl F
FAU - Hameister, H
AU  - Hameister H
FAU - Varga, G
AU  - Varga G
FAU - Friess, H
AU  - Friess H
FAU - Buchler, M
AU  - Buchler M
FAU - Beger, H G
AU  - Beger HG
FAU - Vila, M R
AU  - Vila MR
FAU - Adler, G
AU  - Adler G
FAU - Gress, T M
AU  - Gress TM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Oncogene
JT  - Oncogene
JID - 8711562
RN  - 0 (DNA, Complementary)
RN  - 0 (IGF2BP3 protein, human)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA-Binding Proteins)
SB  - IM
MH  - Amino Acid Sequence
MH  - Base Sequence
MH  - Cloning, Molecular
MH  - DNA, Complementary
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Molecular Sequence Data
MH  - Neoplasm Proteins
MH  - Pancreatic Neoplasms/*genetics
MH  - RNA, Messenger/genetics/metabolism
MH  - RNA-Binding Proteins/*genetics
MH  - Sequence Homology, Amino Acid
MH  - Tumor Cells, Cultured
EDAT- 1997/06/05 00:00
MHDA- 1997/06/05 00:01
CRDT- 1997/06/05 00:00
PHST- 1997/06/05 00:00 [pubmed]
PHST- 1997/06/05 00:01 [medline]
PHST- 1997/06/05 00:00 [entrez]
AID - 10.1038/sj.onc.1201110 [doi]
PST - ppublish
SO  - Oncogene. 1997 Jun 5;14(22):2729-33. doi: 10.1038/sj.onc.1201110.