PMID- 9178833
OWN - NLM
STAT- MEDLINE
DCOM- 19970710
LR  - 20130304
IS  - 0887-6924 (Print)
IS  - 0887-6924 (Linking)
VI  - 11 Suppl 2
DP  - 1997 Apr
TI  - Cytogenetic and molecular cytogenetic analysis of B cell chronic lymphocytic 
      leukemia: specific chromosome aberrations identify prognostic subgroups of 
      patients and point to loci of candidate genes.
PG  - S19-24
AB  - The most frequent chromosome aberrations in B cell chronic lymphocytic leukemia 
      (B-CLL) detected by conventional chromosome banding analysis are trisomy 12 
      followed by structural abnormalities of the long arms of chromosomes 13, 14, and 
      11. Complex karyotypes, trisomy 12, and a '14q+' abnormality have been associated 
      with inferior prognosis, whereas aberrations of 13q have been found in patients 
      with a favorable outcome. However, the cytogenetic analysis of B-CLL by 
      conventional banding techniques has remained a difficult task mainly due to the 
      low in vitro mitotic activity of the tumor cells. Although B cell mitogens are 
      used for cell culture, clonal chromosome aberrations are detected in only half of 
      the B-CLL tumors. 'Interphase cytogenetics' by means of fluorescence in situ 
      hybridization (FISH) circumvents this problem, because there is no need to induce 
      the malignant cells to proliferate in vitro. Numerical and structural chromosome 
      aberrations can be detected in non-dividing interphase cells as well as in 
      metaphase spreads. By FISH, the most common chromosome abnormalities are 
      deletions of 13q followed by deletions of 11q, trisomy 12, and deletions of 17p. 
      Except for the TP53 gene at 17p13, no candidate gene affected by these 
      aberrations has so far been identified. FISH will be instrumental for the 
      identification of such genes because the recurrent aberrations, especially 
      deletions, can be systematically delineated to the resolution level of several 
      kb. Furthermore, based on the sensitive detection of chromosome abnormalities by 
      FISH, more accurate correlations between chromosome abnormalities and prognosis 
      can be performed. Deletion of the TP53 gene at 17p13 have already been shown to 
      be one of the most important independent prognostic factors for survival. Other 
      specific aberrations of clinical significance will likely be identified by the 
      systematic application of interphase cytogenetics on a large series of patients.
FAU - Dohner, H
AU  - Dohner H
AD  - Medizinische Klinik and Poliklinik V, Universitat Heidelberg, Germany.
FAU - Stilgenbauer, S
AU  - Stilgenbauer S
FAU - Fischer, K
AU  - Fischer K
FAU - Bentz, M
AU  - Bentz M
FAU - Lichter, P
AU  - Lichter P
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Review
PL  - England
TA  - Leukemia
JT  - Leukemia
JID - 8704895
RN  - 0 (Genetic Markers)
SB  - IM
MH  - *Chromosome Aberrations
MH  - *Chromosome Disorders
MH  - Chromosome Mapping
MH  - Cytogenetics
MH  - Genetic Markers
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Leukemia, Lymphocytic, Chronic, B-Cell/*genetics/*therapy
MH  - Mutation
MH  - Prognosis
RF  - 65
EDAT- 1997/04/01 00:00
MHDA- 1997/04/01 00:01
CRDT- 1997/04/01 00:00
PHST- 1997/04/01 00:00 [pubmed]
PHST- 1997/04/01 00:01 [medline]
PHST- 1997/04/01 00:00 [entrez]
PST - ppublish
SO  - Leukemia. 1997 Apr;11 Suppl 2:S19-24.