PMID- 9178851
OWN - NLM
STAT- MEDLINE
DCOM- 19970730
LR  - 20190701
IS  - 0304-3940 (Print)
IS  - 0304-3940 (Linking)
VI  - 227
IP  - 1
DP  - 1997 May 9
TI  - The angiotensin AT2 receptor down-regulates neurofilament M in PC12W cells.
PG  - 29-32
AB  - The angiotensin (ANG II) AT2 receptor mediates antiproliferative effects and 
      induces neurite outgrowth in PC12W cells. To further investigate the molecular 
      events following AT2 receptor stimulation in these cells, we determined the 
      expression pattern of the middle-sized neurofilament subunit (NF-M) using Western 
      and Northern blot analysis and reverse-transcription polymerase chain reaction. 
      On both, the protein and the mRNA level, ANG II via AT2 receptors not only 
      counteracted nerve growth factor (NGF)-mediated NF-M up-regulation but also 
      reduced NF-M levels in the absence of NGF by maximally 72%. The ANG II-induced 
      effects were completely abolished by pretreatment with the AT2 receptor 
      antagonist, PD123177. In view of previous findings of decreased NF levels in 
      regenerating neurons and in neuronal cultures undergoing apoptosis, our 
      observation suggests a new role of AT2 receptors in either of these processes.
FAU - Gallinat, S
AU  - Gallinat S
AD  - Department of Pharmacology, Christian-Albrechts-University, Kiel, Germany.
FAU - Csikos, T
AU  - Csikos T
FAU - Meffert, S
AU  - Meffert S
FAU - Herdegen, T
AU  - Herdegen T
FAU - Stoll, M
AU  - Stoll M
FAU - Unger, T
AU  - Unger T
LA  - eng
PT  - Journal Article
PL  - Ireland
TA  - Neurosci Lett
JT  - Neuroscience letters
JID - 7600130
RN  - 0 (Angiotensin Receptor Antagonists)
RN  - 0 (Biphenyl Compounds)
RN  - 0 (Imidazoles)
RN  - 0 (Neurofilament Proteins)
RN  - 0 (Receptors, Angiotensin)
RN  - 0 (Tetrazoles)
RN  - JMS50MPO89 (Losartan)
SB  - IM
MH  - Angiotensin Receptor Antagonists
MH  - Animals
MH  - Biphenyl Compounds/pharmacology
MH  - Cell Differentiation/physiology
MH  - Down-Regulation
MH  - Imidazoles/pharmacology
MH  - Linear Models
MH  - Losartan
MH  - Neurofilament Proteins/*metabolism
MH  - PC12 Cells
MH  - Polymerase Chain Reaction/methods
MH  - Rats
MH  - Receptors, Angiotensin/*physiology
MH  - Tetrazoles/pharmacology
MH  - Transcription, Genetic
EDAT- 1997/05/09 00:00
MHDA- 1997/05/09 00:01
CRDT- 1997/05/09 00:00
PHST- 1997/05/09 00:00 [pubmed]
PHST- 1997/05/09 00:01 [medline]
PHST- 1997/05/09 00:00 [entrez]
AID - S0304-3940(97)00291-7 [pii]
AID - 10.1016/s0304-3940(97)00291-7 [doi]
PST - ppublish
SO  - Neurosci Lett. 1997 May 9;227(1):29-32. doi: 10.1016/s0304-3940(97)00291-7.