PMID- 9182671
OWN - NLM
STAT- MEDLINE
DCOM- 19970721
LR  - 20190508
IS  - 0021-9525 (Print)
IS  - 1540-8140 (Electronic)
IS  - 0021-9525 (Linking)
VI  - 137
IP  - 6
DP  - 1997 Jun 16
TI  - The zinc-finger protein slug causes desmosome dissociation, an initial and 
      necessary step for growth factor-induced epithelial-mesenchymal transition.
PG  - 1403-19
AB  - Epithelial-mesenchymal transition (EMT) is an essential morphogenetic process 
      during embryonic development. It can be induced in vitro by hepatocyte growth 
      factor/scatter factor (HGF/SF), or by FGF-1 in our NBT-II cell model for EMT. We 
      tested for a central role in EMT of a zinc-finger protein called Slug. Slug mRNA 
      and protein levels were increased transiently in FGF-1-treated NBT-II cells. 
      Transient or stable transfection of Slug cDNA in NBT-II cells resulted in a 
      striking disappearance of the desmosomal markers desmoplakin and desmoglein from 
      cell-cell contact areas, mimicking the initial steps of FGF-1 or HGF/SF- induced 
      EMT. Stable transfectant cells expressed Slug protein and were less epithelial, 
      with increased cell spreading and cell-cell separation in subconfluent cultures. 
      Interestingly, NBT-II cells transfected with antisense Slug cDNA were able to 
      resist EMT induction by FGF-1 or even HGF/SF. This antisense effect was 
      suppressed by retransfection with Slug sense cDNA. Our results indicate that Slug 
      induces the first phase of growth factor-induced EMT, including desmosome 
      dissociation, cell spreading, and initiation of cell separation. Moreover, the 
      antisense inhibition experiments suggest that Slug is also necessary for EMT.
FAU - Savagner, P
AU  - Savagner P
AD  - Centre National de la Recherche Scientifique-Institut Curie, 75231 Paris Cedex 
      05, France.
FAU - Yamada, K M
AU  - Yamada KM
FAU - Thiery, J P
AU  - Thiery JP
LA  - eng
SI  - GENBANK/U97059
SI  - GENBANK/U97060
SI  - GENBANK/U97061
GR  - 2R01 CA 490417-06/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Cell Biol
JT  - The Journal of cell biology
JID - 0375356
RN  - 0 (Cadherins)
RN  - 0 (DNA, Antisense)
RN  - 0 (DNA, Complementary)
RN  - 0 (RNA, Messenger)
RN  - 0 (SNAI1 protein, human)
RN  - 0 (Snai2 protein, mouse)
RN  - 0 (Snai2 protein, rat)
RN  - 0 (Snail Family Transcription Factors)
RN  - 0 (Transcription Factors)
RN  - 104781-85-3 (Fibroblast Growth Factor 1)
RN  - 67256-21-7 (Hepatocyte Growth Factor)
RN  - 68238-35-7 (Keratins)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Cadherins/metabolism
MH  - Cell Movement
MH  - Chickens
MH  - Cloning, Molecular
MH  - DNA, Antisense
MH  - DNA, Complementary
MH  - Desmosomes/drug effects/*physiology
MH  - Epithelium/drug effects
MH  - Fibroblast Growth Factor 1/*pharmacology
MH  - Gene Expression
MH  - Hepatocyte Growth Factor/*pharmacology
MH  - Humans
MH  - Keratins/metabolism
MH  - Mammals
MH  - Mesoderm/*physiology
MH  - Mice
MH  - Microscopy, Fluorescence
MH  - Molecular Sequence Data
MH  - Phenotype
MH  - RNA, Messenger
MH  - Rats
MH  - Sequence Analysis, DNA
MH  - Sequence Homology, Amino Acid
MH  - Snail Family Transcription Factors
MH  - Transcription Factors/genetics/*metabolism
MH  - Transfection
MH  - Tumor Cells, Cultured
MH  - Video Recording
MH  - *Zinc Fingers
PMC - PMC2132541
EDAT- 1997/06/16 00:00
MHDA- 1997/06/16 00:01
PMCR- 1997/12/16
CRDT- 1997/06/16 00:00
PHST- 1997/06/16 00:00 [pubmed]
PHST- 1997/06/16 00:01 [medline]
PHST- 1997/06/16 00:00 [entrez]
PHST- 1997/12/16 00:00 [pmc-release]
AID - 10.1083/jcb.137.6.1403 [doi]
PST - ppublish
SO  - J Cell Biol. 1997 Jun 16;137(6):1403-19. doi: 10.1083/jcb.137.6.1403.