PMID- 9184665
OWN - NLM
STAT- MEDLINE
DCOM- 19970627
LR  - 20041117
IS  - 0022-3069 (Print)
IS  - 0022-3069 (Linking)
VI  - 56
IP  - 6
DP  - 1997 Jun
TI  - Detection of malignant cells in cerebrospinal fluid using fluorescence in situ 
      hybridization.
PG  - 743-8
AB  - Cytologic examination of cerebrospinal fluid (CSF) is the diagnostic gold 
      standard for leptomeningeal metastasis (LMM). However, this technique is only 
      moderately sensitive when routine staining procedures are applied. The use of 
      fluorescence in situ hybridization (FISH) to identify malignant cells may have an 
      additional value in diagnosing LMM, since numerical chromosomal aberrations (NCA) 
      can be detected at the single cell level. We tested the feasibility of FISH to 
      detect tumor cells in CSF by analyzing 22 samples with proven LMM with a probe 
      for chromosome 1 (1q12) to detect NCA in the cells. A control group consisted of 
      samples from 10 patients with inflammatory neurologic disease. In 7 LMM samples 
      no cells or only lysed cells were found, due to time delay before fixation. Of 
      the other 15 LMM samples analyzed, 13 showed NCA (87%), while no NCA were found 
      in the control group. Our results indicate that FISH may be a useful additional 
      diagnostic tool to the cytodiagnosis of CSF in cases of LMM. We expect that FISH 
      can become an additional marker for malignancy at the single cell level in 
      patients with LMM, which may also be of use to determine the effect of therapy 
      for LMM.
FAU - van Oostenbrugge, R J
AU  - van Oostenbrugge RJ
AD  - Department of Neurology, University Hospital Maastricht, The Netherlands.
FAU - Hopman, A H
AU  - Hopman AH
FAU - Lenders, M H
AU  - Lenders MH
FAU - van Heerde, P
AU  - van Heerde P
FAU - Arends, J W
AU  - Arends JW
FAU - Ramaekers, F C
AU  - Ramaekers FC
FAU - Twijnstra, A
AU  - Twijnstra A
LA  - eng
PT  - Journal Article
PL  - England
TA  - J Neuropathol Exp Neurol
JT  - Journal of neuropathology and experimental neurology
JID - 2985192R
SB  - IM
MH  - *Arachnoid
MH  - Chromosome Aberrations
MH  - Chromosomes, Human, Pair 1
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Meningeal Neoplasms/*cerebrospinal fluid/*secondary
MH  - *Pia Mater
EDAT- 1997/06/01 00:00
MHDA- 1997/06/01 00:01
CRDT- 1997/06/01 00:00
PHST- 1997/06/01 00:00 [pubmed]
PHST- 1997/06/01 00:01 [medline]
PHST- 1997/06/01 00:00 [entrez]
PST - ppublish
SO  - J Neuropathol Exp Neurol. 1997 Jun;56(6):743-8.