PMID- 9186534
OWN - NLM
STAT- MEDLINE
DCOM- 19970707
LR  - 20190909
IS  - 0902-4441 (Print)
IS  - 0902-4441 (Linking)
VI  - 58
IP  - 4
DP  - 1997 Apr
TI  - Differences in cell lineage involvement between MDS-AML and de novo AML studied 
      by fluorescence in situ hybridization in combination with morphology.
PG  - 241-5
AB  - We have employed fluorescence in situ hybridization (FISH) in combination with 
      standard morphology (MGG/FISH) to identify the clonal involvement of different 
      bone marrow cell lineages in 20 AML patients (14 MDS-AML, 6 de novo AML). Even 
      though the number of cells belonging to the abnormal clone varied between 
      individual cases, the percentage of clonal blasts was similar in MDS-AML and de 
      novo AML patients. The erythropoietic cells appeared to be part of the abnormal 
      clone in 13 of 14 patients with MDS-AML, but only in 1 of 6 with de novo AML. 
      Similarly, clonal granulocytes were detected in 13 of 14 patients with MDS-AML, 
      compared to 2 of 6 with de novo AML. Lymphocytes consistently displayed normal, 
      diploid karyotype. The results suggest that it is possible to distinguish between 
      MDS-AML and de novo AML by the use of MGG/FISH; in de novo AML the abnormal 
      chromosomal clone is generally confined to the immature myeloid cells, while in 
      MDS-AML mature granulocytes and erythroid cells are of clonal origin. It is, 
      however, not possible to conclude that MDS-AML is a "multipotent" type of 
      leukaemia, since it cannot be ruled out that the chromosomally aberrant erythroid 
      cells and granulocytes represent surviving cells from the original MDS clone.
FAU - Bernell, P
AU  - Bernell P
AD  - Department of Medicine, Karolinska Institute at Danderyd Hospital, Stockholm, 
      Sweden.
FAU - Arvidsson, I
AU  - Arvidsson I
FAU - Hast, R
AU  - Hast R
FAU - Jacobsson, B
AU  - Jacobsson B
FAU - Stenke, L
AU  - Stenke L
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Eur J Haematol
JT  - European journal of haematology
JID - 8703985
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Bone Marrow/*pathology
MH  - Chromosomes, Human, Pair 7
MH  - Female
MH  - Granulocytes/pathology
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Interphase
MH  - Karyotyping
MH  - Leukemia, Monocytic, Acute/blood/classification/*genetics/*pathology
MH  - Male
MH  - Middle Aged
MH  - Myelodysplastic Syndromes/blood/*genetics/*pathology
EDAT- 1997/04/01 00:00
MHDA- 1997/04/01 00:01
CRDT- 1997/04/01 00:00
PHST- 1997/04/01 00:00 [pubmed]
PHST- 1997/04/01 00:01 [medline]
PHST- 1997/04/01 00:00 [entrez]
AID - 10.1111/j.1600-0609.1997.tb01661.x [doi]
PST - ppublish
SO  - Eur J Haematol. 1997 Apr;58(4):241-5. doi: 10.1111/j.1600-0609.1997.tb01661.x.