PMID- 9186940
OWN - NLM
STAT- MEDLINE
DCOM- 19970731
LR  - 20190831
IS  - 0166-0934 (Print)
IS  - 0166-0934 (Linking)
VI  - 65
IP  - 2
DP  - 1997 May
TI  - An indirect ELISA for the detection of antibodies against porcine reproductive 
      and respiratory syndrome virus using recombinant nucleocapsid protein as antigen.
PG  - 169-81
AB  - An indirect enzyme-linked immunosorbent assay termed rnPRRS ELISA using 
      baculovirus-expressed and affinity-purified viral nucleocapsid protein (rNC) 
      antigen was developed for detecting antibodies against porcine reproductive and 
      respiratory syndrome virus (PRRSV) in swine sera. Sera (1395) originating from 
      different European countries were used for the validation of this assay. The 
      rnPRRS ELISA was capable of detecting antibodies in all sera known to contain 
      anti-PRRSV antibodies, resulting in 100% sensitivity. The specificity was 95.8%. 
      The rnPRRS ELISA was more sensitive compared to the most widely used tests for 
      the diagnosis of porcine reproductive and respiratory syndrome (PRRS) (i) a 
      commercially available ELISA; (ii) the indirect immunofluorescence assay (IIFA); 
      and (iii) the immunoperoxidase monolayer assay (IPMA). The main advantage of the 
      rnPRRS ELISA compared to an ELISA using whole virus antigen is the use of a 
      single immunogenic protein instead of infectious PRRSV. The rnPRRS ELISA is 
      suitable for routine diagnosis of PRRS and also for epidemiological surveys since 
      it allows highly reliable testing of a large number of sera within a short period 
      of time.
FAU - Denac, H
AU  - Denac H
AD  - Institute of Virology and Immunoprophylaxis, Mittelhausern, Switzerland.
FAU - Moser, C
AU  - Moser C
FAU - Tratschin, J D
AU  - Tratschin JD
FAU - Hofmann, M A
AU  - Hofmann MA
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - J Virol Methods
JT  - Journal of virological methods
JID - 8005839
RN  - 0 (Antibodies, Viral)
RN  - 0 (Antigens, Viral)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Viral Structural Proteins)
SB  - IM
MH  - Animals
MH  - Antibodies, Viral/*blood
MH  - Antigen-Antibody Reactions
MH  - Antigens, Viral/*genetics
MH  - Baculoviridae/chemistry/genetics
MH  - Cloning, Molecular
MH  - Enzyme-Linked Immunosorbent Assay/methods
MH  - Genes, Viral
MH  - Genetic Vectors
MH  - Macrophages, Alveolar/virology
MH  - Nucleocapsid/*genetics/*immunology/metabolism
MH  - Polymerase Chain Reaction
MH  - Porcine respiratory and reproductive syndrome virus/*immunology/metabolism
MH  - Recombinant Proteins/*immunology/isolation & purification
MH  - Sensitivity and Specificity
MH  - Sequence Analysis, DNA
MH  - Serology
MH  - Swine
MH  - Viral Structural Proteins/genetics
EDAT- 1997/05/01 00:00
MHDA- 1997/05/01 00:01
CRDT- 1997/05/01 00:00
PHST- 1997/05/01 00:00 [pubmed]
PHST- 1997/05/01 00:01 [medline]
PHST- 1997/05/01 00:00 [entrez]
AID - S0166-0934(97)02186-1 [pii]
AID - 10.1016/s0166-0934(97)02186-1 [doi]
PST - ppublish
SO  - J Virol Methods. 1997 May;65(2):169-81. doi: 10.1016/s0166-0934(97)02186-1.