PMID- 9187501
OWN - NLM
STAT- MEDLINE
DCOM- 19970725
LR  - 20190706
IS  - 0009-8981 (Print)
IS  - 0009-8981 (Linking)
VI  - 261
IP  - 1
DP  - 1997 May 6
TI  - Actin polymerisation in neutrophils of rheumatoid arthritis patients in relation 
      to treatment with non-steroidal anti-inflammatory drugs.
PG  - 19-25
AB  - There is evidence that neutrophil functions such as chemotaxis and oxygen radical 
      formation are disturbed in rheumatoid arthritis (RA). Medication might also 
      influence these functions. Cyclic formation and depolymerisation of actin 
      microfilaments is crucial in cell motility, but this phenomenon has not been 
      studied in RA. The aim of this study was to investigate basal and dynamic 
      (formyl-methionyl-leucyl-phenylalanine (fMLP)-induced) neutrophil actin 
      polymerisation in ten RA patients (a) during therapy with non-steroidal 
      anti-inflammatory drugs (NSAIDS) and (b) after stopping NSAIDS> The results were 
      compared with those of ten age-matched controls. Basal F-actin content in RA 
      patients with NSAIDS was significantly lower than in RA patients without NSAIDS 
      and controls: 35.5 (25.0-49.0), 50.5 (27.0-75.0) and 52.5 (32.0-85.0), 
      respectively. Conversely, upon stimulation with fMLP, the actin polymerisation 
      curve of RA patients with NSAIDS was higher than for RA patients without NSAIDS 
      and controls. These results suggest that, in RA, the effects orf NSAIDS on 
      neutrophil functions might be related to changes in the actin 
      polymerisation-depolymerisation cycle.
FAU - De Clerck, L S
AU  - De Clerck LS
AD  - Department of Immunology, Allergology and Rheumatology, University of Antwerpen, 
      Belgium.
FAU - Mertens, A V
AU  - Mertens AV
FAU - De Gendt, C M
AU  - De Gendt CM
FAU - Bridts, C H
AU  - Bridts CH
FAU - Stevens, W J
AU  - Stevens WJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Clin Chim Acta
JT  - Clinica chimica acta; international journal of clinical chemistry
JID - 1302422
RN  - 0 (Actins)
RN  - 0 (Anti-Inflammatory Agents, Non-Steroidal)
RN  - 0 (Biopolymers)
RN  - 3XC8GUZ6CB (Sulfasalazine)
RN  - 59880-97-6 (N-Formylmethionine Leucyl-Phenylalanine)
SB  - IM
MH  - Actins/blood/*drug effects/*metabolism
MH  - Anti-Inflammatory Agents, Non-Steroidal/*therapeutic use
MH  - Arthritis, Rheumatoid/*blood/drug therapy/metabolism
MH  - Biopolymers/blood
MH  - Drug Synergism
MH  - Humans
MH  - N-Formylmethionine Leucyl-Phenylalanine/pharmacology
MH  - Neutrophils/*chemistry/drug effects
MH  - Sulfasalazine/pharmacology
EDAT- 1997/05/06 00:00
MHDA- 1997/05/06 00:01
CRDT- 1997/05/06 00:00
PHST- 1997/05/06 00:00 [pubmed]
PHST- 1997/05/06 00:01 [medline]
PHST- 1997/05/06 00:00 [entrez]
AID - S0009898196065059 [pii]
AID - 10.1016/s0009-8981(96)06505-9 [doi]
PST - ppublish
SO  - Clin Chim Acta. 1997 May 6;261(1):19-25. doi: 10.1016/s0009-8981(96)06505-9.