PMID- 9187736
OWN - NLM
STAT- MEDLINE
DCOM- 19970819
LR  - 20191102
IS  - 1052-0295 (Print)
IS  - 1052-0295 (Linking)
VI  - 72
IP  - 3
DP  - 1997 May
TI  - Rapid denaturation improves chromosome morphology and permits multiple 
      hybridizations during fluorescence in situ hybridization.
PG  - 141-3
AB  - Denaturation of chromosomal DNA for fluorescence in situ hybridization (FISH) is 
      an essential step in a procedure associated with a number of variables. In our 
      experience, shorter denaturation time in 70% formamide/2 x SSC at 72 C provides 
      sufficient denaturation, where the hydrogen bonds are broken between the purines 
      and pyrimidines of the double helix. This shortened exposure improves retention 
      of morphology of human chromosomes from lymphocytes, aminocytes, fibroblasts and 
      bone marrow, and allows the same metaphases to be denatured repeatedly and 
      rehybridized with different probes. This approach is useful in investigations 
      where sample volume is limited.
FAU - Ramesh, K H
AU  - Ramesh KH
AD  - Division of Genetics, Long Island College Hospital-SUNY Health Science Center at 
      Brooklyn 11201, USA.
FAU - Macera, M J
AU  - Macera MJ
FAU - Verma, R S
AU  - Verma RS
LA  - eng
PT  - Journal Article
PL  - England
TA  - Biotech Histochem
JT  - Biotechnic & histochemistry : official publication of the Biological Stain 
      Commission
JID - 9107378
RN  - 0 (Fixatives)
RN  - 0 (Formamides)
SB  - IM
MH  - *Chromosomes, Human
MH  - Fixatives
MH  - Formamides
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - *Nucleic Acid Denaturation
MH  - Time Factors
EDAT- 1997/05/01 00:00
MHDA- 1997/05/01 00:01
CRDT- 1997/05/01 00:00
PHST- 1997/05/01 00:00 [pubmed]
PHST- 1997/05/01 00:01 [medline]
PHST- 1997/05/01 00:00 [entrez]
AID - 10.3109/10520299709082227 [doi]
PST - ppublish
SO  - Biotech Histochem. 1997 May;72(3):141-3. doi: 10.3109/10520299709082227.