PMID- 9189830
OWN - NLM
STAT- MEDLINE
DCOM- 19970724
LR  - 20190909
IS  - 0021-9304 (Print)
IS  - 0021-9304 (Linking)
VI  - 35
IP  - 4
DP  - 1997 Jun 15
TI  - Inhibition of smooth muscle cell growth in vitro by an antisense 
      oligodeoxynucleotide released from poly(DL-lactic-co-glycolic acid) 
      microparticles.
PG  - 525-30
AB  - We fabricated poly(DL-lactic-co-glycolic acid) (PLGA) 50:50 microparticles loaded 
      with an antisense (AS) oligodeoxy-nucleotide (ODN) against the rat tenascin mRNA 
      and determined the effect in vitro of the AS-ODN released on smooth muscle cell 
      (SMC) proliferation and migration. AS-ODN was entrapped using a 
      double-emulsion-solvent-extraction technique with high efficiency. Release of 
      AS-ODN was characterized by a small initial-burst effect followed by a period of 
      controlled AS-ODN release for up to 20 days. SMC proliferation studies exhibited 
      dose-dependent growth inhibition with AS-ODN-loaded microparticles. 
      Microparticles loaded with scrambled (SC) ODN showed less growth inhibition than 
      AS-ODN. Moreover, only the AS-ODN-loaded microparticles inhibited migration. 
      These results demonstrate the feasibility of entrapping an AS-ODN to rat tenascin 
      in PLGA microparticles for controlled delivery to inhibit SMC proliferation and 
      migration.
FAU - Cleek, R L
AU  - Cleek RL
AD  - Cox Laboratory for Biomedical Engineering, Department of Chemical Engineering, 
      Rice University, Houston, Texas 77251-1892, USA.
FAU - Rege, A A
AU  - Rege AA
FAU - Denner, L A
AU  - Denner LA
FAU - Eskin, S G
AU  - Eskin SG
FAU - Mikos, A G
AU  - Mikos AG
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biomed Mater Res
JT  - Journal of biomedical materials research
JID - 0112726
RN  - 0 (Drug Carriers)
RN  - 0 (Oligonucleotides, Antisense)
RN  - 0 (Polymers)
RN  - 0 (RNA, Messenger)
RN  - 0 (Tenascin)
RN  - 12770-88-6 (polysorb)
SB  - IM
MH  - Animals
MH  - Cell Division/*drug effects
MH  - Cell Movement/drug effects
MH  - Drug Carriers
MH  - Microspheres
MH  - Muscle, Smooth, Vascular/cytology/*drug effects
MH  - Oligonucleotides, Antisense/administration & 
      dosage/pharmacokinetics/*pharmacology
MH  - *Polymers
MH  - RNA, Messenger/genetics
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Tenascin/genetics
OTO - NASA
OT  - Non-programmatic
EDAT- 1997/06/15 00:00
MHDA- 2000/06/20 09:00
CRDT- 1997/06/15 00:00
PHST- 1997/06/15 00:00 [pubmed]
PHST- 2000/06/20 09:00 [medline]
PHST- 1997/06/15 00:00 [entrez]
AID - 10.1002/(SICI)1097-4636(19970615)35:4<525::AID-JBM12>3.0.CO;2-A [pii]
AID - 10.1002/(sici)1097-4636(19970615)35:4<525::aid-jbm12>3.0.co;2-a [doi]
PST - ppublish
SO  - J Biomed Mater Res. 1997 Jun 15;35(4):525-30. doi: 
      10.1002/(sici)1097-4636(19970615)35:4<525::aid-jbm12>3.0.co;2-a.