PMID- 9191518
OWN - NLM
STAT- MEDLINE
DCOM- 19970626
LR  - 20190620
IS  - 0008-543X (Print)
IS  - 0008-543X (Linking)
VI  - 79
IP  - 12
DP  - 1997 Jun 15
TI  - Detection of numeric abnormalities of chromosome 17 and p53 deletions by 
      fluorescence in situ hybridization in pleomorphic adenomas and carcinomas in 
      pleomorphic adenoma. Correlation with p53 expression.
PG  - 2314-9
AB  - BACKGROUND: A fluorescence in situ hybridization (FISH) technique using specific 
      DNA probes allows for the detection of chromosomal aberrations and gene deletions 
      and gains, even in interphase nuclei in human solid tumors. A high frequency of 
      aberrations of chromosome 17 and mutation of the p53 gene have been reported in 
      some human tumors. The correlation of p53 expression with abnormalities of 
      chromosome 17 and p53 gene deletion in salivary gland tumors has not yet been 
      investigated. METHODS: The authors analyzed the numeric aberrations of chromosome 
      17 and p53 gene deletions in 11 paraffin embedded pleomorphic adenomas (PA) and 9 
      carcinomas in pleomorphic adenoma (CIPA), using FISH techniques. The centromere 
      specific DNA probe for chromosome 17 and p53 cosmid DNA probe was used. The 
      aberrations of chromosome 17 and p53 deletion were correlated with 
      immunohistochemical detection of p53 protein. RESULTS: Monosomy 17 was detected 
      in 30.8% of CIPA cells and 29.6% of PA cells, and polysomy 17 was detected in 
      19.6% of CIPA cells and 9.6% of PA cells. p53 protein expression was observed in 
      6 of 9 CIPA specimens (66.7%) and 2 of 75 PA specimens (2.7%). Deletion of the 
      p53 gene was frequent in p53 protein positive specimens. A statistically 
      significant correlation existed between p53 protein expression and polysomy 17 (P 
      = 0.0417). CONCLUSIONS: It was observed that loss of chromosome 17 may occur in 
      PA before its transformation to carcinoma. p53 expression was frequently 
      associated with deletion of the p53 gene as detected by FISH. Polysomy 17 was 
      more frequent in CIPA than PA and was associated with mutation of p53.
FAU - Li, X
AU  - Li X
AD  - Department of Oral and Maxillofacial Surgery, Yamaguchi University School of 
      Medicine, Ube, Japan.
FAU - Tsuji, T
AU  - Tsuji T
FAU - Wen, S
AU  - Wen S
FAU - Mimura, Y
AU  - Mimura Y
FAU - Sasaki, K
AU  - Sasaki K
FAU - Shinozaki, F
AU  - Shinozaki F
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cancer
JT  - Cancer
JID - 0374236
SB  - IM
MH  - Adenocarcinoma/*genetics
MH  - Adenoma, Pleomorphic/*genetics
MH  - Chromosome Aberrations/*genetics
MH  - Chromosomes, Human, Pair 17/*genetics
MH  - *Gene Deletion
MH  - Genes, p53/*genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Salivary Gland Neoplasms/*genetics
EDAT- 1997/06/15 00:00
MHDA- 2000/06/20 09:00
CRDT- 1997/06/15 00:00
PHST- 1997/06/15 00:00 [pubmed]
PHST- 2000/06/20 09:00 [medline]
PHST- 1997/06/15 00:00 [entrez]
AID - 10.1002/(SICI)1097-0142(19970615)79:12<2314::AID-CNCR4>3.0.CO;2-L [pii]
AID - 10.1002/(sici)1097-0142(19970615)79:12<2314::aid-cncr4>3.0.co;2-l [doi]
PST - ppublish
SO  - Cancer. 1997 Jun 15;79(12):2314-9. doi: 
      10.1002/(sici)1097-0142(19970615)79:12<2314::aid-cncr4>3.0.co;2-l.