PMID- 9191610
OWN - NLM
STAT- MEDLINE
DCOM- 19970626
LR  - 20220410
IS  - 0146-0404 (Print)
IS  - 0146-0404 (Linking)
VI  - 38
IP  - 7
DP  - 1997 Jun
TI  - Flow cytometry in impression cytology specimens. A new method for evaluation of 
      conjunctival inflammation.
PG  - 1458-64
AB  - PURPOSE: To investigate feasibility and potential uses of flow cytometry in 
      impression cytology as a new procedure to assess and quantify conjunctival 
      inflammation. METHODS: Specimens for cytology were collected by impression from 
      30 patients with various chronic ocular surface disorders and from 10 normal 
      subjects. Two specimens were obtained in each eye: One was transferred onto a 
      glass slide and processed by immunofluorescence with antibodies to human 
      leukocyte antigen (HLA)-DR antigens; cells from the other were suspended in 
      phosphate-buffered saline for flow cytometry. Monoclonal antibodies to HLA-DR 
      antigens and CD23, the low affinity receptor to immunoglobulin E, were used. 
      RESULTS: Abnormal expression of HLA-DR and CD23 by conjunctival cells was found 
      in 13 of 18 dry eyes and in 20 of 22 eyes with chronic conjunctivitis, whereas 
      specimens remained almost negative (less than 10% of cells were positive) in 
      normal eyes. Percentages of positive cells ranged between 20% and 98% of all 
      conjunctival cells. Correlation between the two methods, immunocytology and flow 
      cytometry, was highly significant (coefficient of correlation 0.77, P = 0.0001). 
      Moreover, HLA-DR positivity, at its strongest intensity, was observed in a 
      minority of cells (1% to 12%), most of which were resident class II-expressing 
      dendritic cells. Percentages of those cells expressing high levels of HLA-DR were 
      3 +/- 1.2% in normal eyes, 5.8 +/- 4% in dry eyes (P = 0.05), and 5.9 +/- 3.5% in 
      eyes with chronic conjunctivitis (P = 0.02). CONCLUSIONS: Results of this 
      preliminary study confirm that conjunctival epithelial cells may abnormally 
      express inflammatory markers in chronic ocular surface disorders. Development of 
      flow cytometry in analysis of cytologic specimens provides a new, sensitive, and 
      objective tool for exploring conjunctival pathology.
FAU - Baudouin, C
AU  - Baudouin C
AD  - Department of Ophthalmology, Ambroise Pare Hospital, University of Paris-V Rene 
      Descartes, France.
FAU - Brignole, F
AU  - Brignole F
FAU - Becquet, F
AU  - Becquet F
FAU - Pisella, P J
AU  - Pisella PJ
FAU - Goguel, A
AU  - Goguel A
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Invest Ophthalmol Vis Sci
JT  - Investigative ophthalmology & visual science
JID - 7703701
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (HLA-DR Antigens)
RN  - 0 (Receptors, IgE)
SB  - IM
MH  - Antibodies, Monoclonal
MH  - Conjunctiva/metabolism/pathology
MH  - Conjunctivitis/metabolism/*pathology
MH  - Dry Eye Syndromes/pathology
MH  - Epithelium/metabolism/pathology
MH  - Flow Cytometry/*methods
MH  - Fluorescent Antibody Technique, Indirect
MH  - HLA-DR Antigens/metabolism
MH  - Humans
MH  - Receptors, IgE/metabolism
EDAT- 1997/06/01 00:00
MHDA- 1997/06/01 00:01
CRDT- 1997/06/01 00:00
PHST- 1997/06/01 00:00 [pubmed]
PHST- 1997/06/01 00:01 [medline]
PHST- 1997/06/01 00:00 [entrez]
PST - ppublish
SO  - Invest Ophthalmol Vis Sci. 1997 Jun;38(7):1458-64.