PMID- 9191863
OWN - NLM
STAT- MEDLINE
DCOM- 19970707
LR  - 20190815
IS  - 0304-8608 (Print)
IS  - 0304-8608 (Linking)
VI  - 142
IP  - 5
DP  - 1997
TI  - Genetic variation and phylogenetic relationships of 22 porcine reproductive and 
      respiratory syndrome virus (PRRSV) field strains based on sequence analysis of 
      open reading frame 5.
PG  - 993-1001
AB  - Porcine reproductive and respiratory syndrome virus (PRRSV) strains from 13 
      states in the United States, Guatemala and Canada were used to compare the 
      envelope glycoprotein gene (ORF 5) nucleotide and deduced amino acid sequences. 
      The gene was the same size, 603 nt, for all the 22 field strains. These strains 
      had 89-94% amino acid identity compared to reference strain VR 2332. A putative 
      signal sequence and cleavage site between residues 31 and 32 was identified and 
      three potential glycosylation sites were present on all but two strains. 
      Hydrophobicity/hydrophilicity and surface probability analyses reveal a primary 
      structure for the envelope glycoprotein (E protein) with six potential surface 
      regions that could be antigenic sites. Similar E protein structural features are 
      conserved for the prototype European PRRSV-Lelystad virus.
FAU - Andreyev, V G
AU  - Andreyev VG
AD  - Virology Swine Research Unit, USDA, Ames, Iowa, USA.
FAU - Wesley, R D
AU  - Wesley RD
FAU - Mengeling, W L
AU  - Mengeling WL
FAU - Vorwald, A C
AU  - Vorwald AC
FAU - Lager, K M
AU  - Lager KM
LA  - eng
SI  - GENBANK/U66378
SI  - GENBANK/U66379
SI  - GENBANK/U66380
SI  - GENBANK/U66381
SI  - GENBANK/U66382
SI  - GENBANK/U66383
SI  - GENBANK/U66384
SI  - GENBANK/U66385
SI  - GENBANK/U66386
SI  - GENBANK/U66387
SI  - GENBANK/U66388
SI  - GENBANK/U66389
SI  - GENBANK/U66390
SI  - GENBANK/U66391
SI  - GENBANK/U66392
SI  - GENBANK/U66393
SI  - GENBANK/U66394
SI  - GENBANK/U66395
SI  - GENBANK/U66396
SI  - GENBANK/U66397
SI  - GENBANK/U66398
SI  - GENBANK/U66399
PT  - Journal Article
PL  - Austria
TA  - Arch Virol
JT  - Archives of virology
JID - 7506870
RN  - 0 (Viral Envelope Proteins)
SB  - IM
MH  - Amino Acid Sequence
MH  - Genetic Variation
MH  - Molecular Sequence Data
MH  - Open Reading Frames
MH  - Phylogeny
MH  - Porcine respiratory and reproductive syndrome virus/*genetics/isolation & 
      purification
MH  - Viral Envelope Proteins/genetics
EDAT- 1997/01/01 00:00
MHDA- 1997/01/01 00:01
CRDT- 1997/01/01 00:00
PHST- 1997/01/01 00:00 [pubmed]
PHST- 1997/01/01 00:01 [medline]
PHST- 1997/01/01 00:00 [entrez]
AID - 10.1007/s007050050134 [doi]
PST - ppublish
SO  - Arch Virol. 1997;142(5):993-1001. doi: 10.1007/s007050050134.