PMID- 9191896
OWN - NLM
STAT- MEDLINE
DCOM- 19970703
LR  - 20190909
IS  - 0955-3002 (Print)
IS  - 0955-3002 (Linking)
VI  - 71
IP  - 5
DP  - 1997 May
TI  - Whole library-amplification and labelling of human chromosome-specific composite 
      probes for fluorescence in situ hybridization (FISH) using PCR.
PG  - 515-7
AB  - Chromosome painting with human-specific, whole-plasmid library probes has become 
      a widespread technique in cytogenetic research and radiation biology. Fast and 
      convenient methods for probe amplification and labelling are required that 
      conserve the complexity of the libraries and stain the entire length of selected 
      chromosomes. The present study uses PCR for amplification and labelling of the 
      whole bluescribe plasmid libraries pBS1, pBS4 and pBS12 using M13 forward and 
      reverse sequencing primer. Amplification and labelling can be accomplished within 
      a few hours with a minimum of laboratory equipment and costs. It is therefore 
      suitable for all laboratories that do not have the facilities for growing 
      transformed bacteria containing plasmids with inserts of human origin.
FAU - Oberheitmann, B
AU  - Oberheitmann B
AD  - Department of Environmental Epidemiology, University of Bremen, Germany.
LA  - eng
PT  - Journal Article
PL  - England
TA  - Int J Radiat Biol
JT  - International journal of radiation biology
JID - 8809243
RN  - 0 (DNA Probes)
SB  - IM
MH  - *Chromosome Aberrations
MH  - DNA Probes
MH  - Gene Library
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - *Polymerase Chain Reaction
EDAT- 1997/05/01 00:00
MHDA- 1997/05/01 00:01
CRDT- 1997/05/01 00:00
PHST- 1997/05/01 00:00 [pubmed]
PHST- 1997/05/01 00:01 [medline]
PHST- 1997/05/01 00:00 [entrez]
AID - 10.1080/095530097143842 [doi]
PST - ppublish
SO  - Int J Radiat Biol. 1997 May;71(5):515-7. doi: 10.1080/095530097143842.