PMID- 9191924 OWN - NLM STAT- MEDLINE DCOM- 19970703 LR - 20061115 IS - 0022-1317 (Print) IS - 0022-1317 (Linking) VI - 78 ( Pt 6) DP - 1997 Jun TI - Nucleotide sequence of the glycoprotein gene of viral haemorrhagic septicaemia (VHS) viruses from different geographical areas: a link between VHS in farmed fish species and viruses isolated from North Sea cod (Gadus morhua L.). PG - 1319-26 AB - RT-PCR methods have been applied to the detection and sequencing of the glycoprotein gene of viral haemorrhagic septicaemia virus (VHSV), the rhabdovirus which causes viral haemorrhagic septicaemia (VHS) in farmed salmonid fish. Phylogenetic analysis of a 360 nt region of the glycoprotein gene from a range of marine and fresh water VHSV isolates identified three genogroups, I-III. Significantly, two virus isolates recovered from ulcerated North Sea cod caught off the Shetland Islands, and an isolate recovered from diseased turbot farmed on the island of Gigha, Scotland were assigned to the same genogroup. Moreover, a virus isolated from diseased turbot farmed on the Baltic Sea coast shared 99.4% nucleotide sequence similarity with a virus associated with a VHS outbreak in rainbow trout. This is the first time that a genetic link between a VHS outbreak and natural VHSV infections of marine fish species has been demonstrated. FAU - Stone, D M AU - Stone DM AD - Centre for Environment Fisheries and Aquaculture Science, Weymouth Laboratory, Dorset, UK. d.m.stone@cefas.co.uk FAU - Way, K AU - Way K FAU - Dixon, P F AU - Dixon PF LA - eng SI - GENBANK/Z93404 SI - GENBANK/Z93405 SI - GENBANK/Z93406 SI - GENBANK/Z93407 SI - GENBANK/Z93408 SI - GENBANK/Z93409 SI - GENBANK/Z93410 SI - GENBANK/Z93411 SI - GENBANK/Z93412 SI - GENBANK/Z93413 SI - GENBANK/Z93414 SI - GENBANK/Z93415 SI - GENBANK/Z93416 SI - GENBANK/Z93417 SI - GENBANK/Z93418 SI - GENBANK/Z93419 SI - GENBANK/Z93420 SI - GENBANK/Z93421 SI - GENBANK/Z93422 SI - GENBANK/Z93423 SI - GENBANK/Z93424 SI - GENBANK/Z93425 SI - GENBANK/Z93426 SI - GENBANK/Z93427 SI - GENBANK/Z93428 SI - GENBANK/Z93429 SI - GENBANK/Z93430 SI - GENBANK/Z93431 PT - Journal Article PL - England TA - J Gen Virol JT - The Journal of general virology JID - 0077340 RN - 0 (Glycoproteins) SB - IM MH - Amino Acid Sequence MH - Animals MH - Base Sequence MH - Fishes/*virology MH - Glycoproteins/*genetics MH - Hemorrhage/*virology MH - Molecular Sequence Data MH - Rhabdoviridae/*genetics MH - Sepsis/*virology EDAT- 1997/06/01 00:00 MHDA- 1997/06/01 00:01 CRDT- 1997/06/01 00:00 PHST- 1997/06/01 00:00 [pubmed] PHST- 1997/06/01 00:01 [medline] PHST- 1997/06/01 00:00 [entrez] AID - 10.1099/0022-1317-78-6-1319 [doi] PST - ppublish SO - J Gen Virol. 1997 Jun;78 ( Pt 6):1319-26. doi: 10.1099/0022-1317-78-6-1319.