PMID- 9192166
OWN - NLM
STAT- MEDLINE
DCOM- 19970825
LR  - 20190831
IS  - 0271-3683 (Print)
IS  - 0271-3683 (Linking)
VI  - 16
IP  - 6
DP  - 1997 Jun
TI  - Effect of mitomycin-C on human retinal pigment epithelium in culture.
PG  - 572-6
AB  - PURPOSE: To determine the effect of mitomycin-C on confluent and non-confluent 
      human retinal pigment epithelium (RPE) in tissue culture. METHODS: The effect of 
      mitomycin-C on confluent RPE was determined by treating first passage confluent 
      cells with 0.01, 0.1, 1, 10, 100 or 1000 micromolar (microM) mitomycin-C for 1, 
      3, or 7 days. The cell viability after treatment was determined by using an 
      esterase stain. The effect of mitomycin-C on proliferating RPE was determined by 
      incubating non-confluent cells with the above concentrations of mitomycin-C for 
      20 min, 1 hour or 24 hours. RESULTS: Mitomycin-C can be toxic to a confluent RPE 
      monolayer, and the LD50 is 421, 28.8 or 0.0632 microM when cells are continually 
      exposed to mitomycin-C for 1, 3 or 7 days, respectively. Exposure to mitomycin-C 
      at concentrations > or = 10 microM for 20-60 min significantly inhibits 
      proliferation of non-confluent RPE. A 24 hour exposure of RPE to 1 microM 
      mitomycin-C markedly inhibits proliferation of non-confluent RPE with minimal 
      toxicity to confluent RPE. CONCLUSIONS: Since exposure of human RPE to 
      mitomycin-C for 24 hours can inhibit cell proliferation at concentrations which 
      are well-tolerated by confluent RPE, mitomycin-C may be a suitable agent for 
      inhibiting RPE proliferation in vivo.
FAU - Ho, T C
AU  - Ho TC
AD  - Department of Ophthalmology and Visual Sciences, School of Medicine, Washington 
      University, St. Louis, MO 63110, USA.
FAU - Del Priore, L V
AU  - Del Priore LV
FAU - Hornbeck, R
AU  - Hornbeck R
LA  - eng
GR  - EY 02687/EY/NEI NIH HHS/United States
GR  - EY 10311/EY/NEI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Curr Eye Res
JT  - Current eye research
JID - 8104312
RN  - 0 (Nucleic Acid Synthesis Inhibitors)
RN  - 50SG953SK6 (Mitomycin)
SB  - IM
MH  - Cell Division/drug effects
MH  - Cells, Cultured
MH  - Humans
MH  - Mitomycin/*pharmacology
MH  - Nucleic Acid Synthesis Inhibitors/*pharmacology
MH  - Pigment Epithelium of Eye/cytology/*drug effects
EDAT- 1997/06/01 00:00
MHDA- 1997/06/01 00:01
CRDT- 1997/06/01 00:00
PHST- 1997/06/01 00:00 [pubmed]
PHST- 1997/06/01 00:01 [medline]
PHST- 1997/06/01 00:00 [entrez]
AID - 10.1076/ceyr.16.6.572.5073 [doi]
PST - ppublish
SO  - Curr Eye Res. 1997 Jun;16(6):572-6. doi: 10.1076/ceyr.16.6.572.5073.