PMID- 9192464
OWN - NLM
STAT- MEDLINE
DCOM- 19970818
LR  - 20190905
IS  - 0045-6535 (Print)
IS  - 0045-6535 (Linking)
VI  - 34
IP  - 11
DP  - 1997 Jun
TI  - Cytochrome P450 3A4 mediated metabolism of 2,4-dichlorophenol.
PG  - 2281-91
AB  - The metabolism of the environmental pollutant and hepatocarcinogen 
      2,4-dichlorophenol (2,4-DCP) was studied using microsomal fractions and 
      whole-cells of Saccharomyces cerevisiae containing human cytochrome P450 3A4. 
      2,4-DCP exhibited a typical type I substrate binding spectrum with a K, of 75 
      microM. 2,4-DCP was metabolised into two major metabolites identified as 
      2-chloro-1,4-hydroxyquinone and 2-chloro-1,4-benzoquinone in microsomal fractions 
      and whole cells of yeast expressing human cytochrome P450 3A4. A further 
      metabolite, 1,2,4-hydroxybenzene, was also detected during biotransformation by 
      whole cells, but was not observed in microsomal fractions. 2,4-DCP metabolism was 
      dependent on NADPH in microsomal fractions and no activity was observed in 
      microsomal fractions or whole cells of control transformants. Metabolites were 
      identified by TLC followed by GC-MS.
FAU - Mehmood, Z
AU  - Mehmood Z
AD  - Krebs Institute for Biomolecular Research, Department of Molecular Biology and 
      Biotechnology, University of Sheffield, UK.
FAU - Kelly, D E
AU  - Kelly DE
FAU - Kelly, S L
AU  - Kelly SL
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Chemosphere
JT  - Chemosphere
JID - 0320657
RN  - 0 (Anthelmintics)
RN  - 0 (Benzoquinones)
RN  - 0 (Chlorophenols)
RN  - 0 (Culture Media)
RN  - 0 (Environmental Pollutants)
RN  - 0 (Hydroquinones)
RN  - 0 (Recombinant Proteins)
RN  - 53-59-8 (NADP)
RN  - 615-67-8 (2-chloro-1,4-hydroxyquinone)
RN  - 695-99-8 (2-chloro-1,4-benzoquinone)
RN  - 9035-51-2 (Cytochrome P-450 Enzyme System)
RN  - EC 1.- (Mixed Function Oxygenases)
RN  - EC 1.14.14.1 (CYP3A protein, human)
RN  - EC 1.14.14.1 (Cytochrome P-450 CYP3A)
RN  - R669TG1950 (2,4-dichlorophenol)
SB  - IM
MH  - Anthelmintics/*metabolism
MH  - Benzoquinones/analysis/metabolism
MH  - Binding Sites
MH  - Biotransformation
MH  - Chlorophenols/*metabolism
MH  - Chromatography, Thin Layer
MH  - Culture Media
MH  - Cytochrome P-450 CYP3A
MH  - Cytochrome P-450 Enzyme System/*metabolism
MH  - Environmental Pollutants/metabolism
MH  - Gas Chromatography-Mass Spectrometry
MH  - Humans
MH  - Hydroquinones/analysis/metabolism
MH  - Microsomes, Liver/enzymology
MH  - Mixed Function Oxygenases/*metabolism
MH  - NADP/metabolism
MH  - Recombinant Proteins/metabolism
MH  - Saccharomyces cerevisiae/enzymology/genetics/growth & development
EDAT- 1997/06/01 00:00
MHDA- 1997/06/01 00:01
CRDT- 1997/06/01 00:00
PHST- 1997/06/01 00:00 [pubmed]
PHST- 1997/06/01 00:01 [medline]
PHST- 1997/06/01 00:00 [entrez]
AID - S0045653597000404 [pii]
AID - 10.1016/s0045-6535(97)00040-4 [doi]
PST - ppublish
SO  - Chemosphere. 1997 Jun;34(11):2281-91. doi: 10.1016/s0045-6535(97)00040-4.