PMID- 9195156
OWN - NLM
STAT- MEDLINE
DCOM- 19970827
LR  - 20220419
IS  - 1018-4813 (Print)
IS  - 1018-4813 (Linking)
VI  - 5
IP  - 2
DP  - 1997 Mar-Apr
TI  - The meiotic segregation pattern of a reciprocal translocation 
      t(10;12)(q26.1;p13.3) by fluorescence in situ hybridization sperm analysis.
PG  - 78-82
AB  - The meiotic segregation of chromosomes 10 and 12 was analyzed in a male 
      heterozygous for a reciprocal translocation, t(10;12)(q26.1;p13.3), using 
      fluorescence in situ hybridization (FISH). Centromeric specific probes that 
      detect alpha satellite sequences of chromosomes 10 and 12 were used. A total of 
      10,049 spermatozoa were analyzed. The frequencies of alternate/adjacent 1, 
      adjacent 2, and 3:1 modes of segregation were: 84.25, 10.95%, and 4.42%, 
      respectively. Diploidy was present in 0.23% of spermatozoa. Similar segregation 
      patterns have been reported for this donor by direct karyotyping of sperm cells. 
      FISH is a valuable technique for studying meiotic segregation patterns in that 
      larger samples can be studied in a relatively short time. However, it does not 
      provide information on the full chromosome complement of the spermatozoon.
FAU - Estop, A M
AU  - Estop AM
AD  - Center for Medical Genetics, Allegheny General Hospital, Pittsburgh, PA 15212, 
      USA. estop@asri.edu
FAU - Cieply, K M
AU  - Cieply KM
FAU - Aston, C E
AU  - Aston CE
LA  - eng
PT  - Journal Article
PL  - England
TA  - Eur J Hum Genet
JT  - European journal of human genetics : EJHG
JID - 9302235
SB  - IM
MH  - Chromosomes, Human, Pair 10/*genetics
MH  - Chromosomes, Human, Pair 12/*genetics
MH  - Heterozygote
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Meiosis
MH  - Spermatozoa/*cytology
MH  - *Translocation, Genetic
EDAT- 1997/03/01 00:00
MHDA- 1997/03/01 00:01
CRDT- 1997/03/01 00:00
PHST- 1997/03/01 00:00 [pubmed]
PHST- 1997/03/01 00:01 [medline]
PHST- 1997/03/01 00:00 [entrez]
PST - ppublish
SO  - Eur J Hum Genet. 1997 Mar-Apr;5(2):78-82.