PMID- 9196436
OWN - NLM
STAT- MEDLINE
DCOM- 19970707
LR  - 20041117
IS  - 0022-3417 (Print)
IS  - 0022-3417 (Linking)
VI  - 181
IP  - 4
DP  - 1997 Apr
TI  - Comparative genomic hybridization (CGH) analysis of neuroblastomas--an important 
      methodological approach in paediatric tumour pathology.
PG  - 394-400
AB  - Comparative genomic hybridization (CGH) was applied to 35 neuroblastomas to 
      obtain a global view of genetic imbalances. Results were validated by means of 
      Southern blot hybridization (detection of N-myc amplification), loss of 
      heterozygosity (LOH) studies (detection of deletion 1p), and interphase 
      cytogenetics [dual labelling fluorescence in situ hybridization (FISH) of 
      centromeric 17 and erbB-2]. CGH allowed sensitive detection of N-myc 
      amplification and chromosome 1p deletion, representing the most established 
      prognostic markers of neuroblastoma. In addition, a high rate of chromosome 17 
      aberrations (63 per cent) with possible prognostic relevance was observed. 
      Previously unreported high level copy number increases indicating oncogene 
      amplification were mapped to chromosome subbands 2p13-14 and 3q24-26. Other 
      recurrent regional chromosomal aberrations were localized on 11q, 12q, 13q, 14q, 
      and 15q. CGH results were fully consistent with data of Southern blot analysis 
      and LOH study, as well as interphase cytogenetics. These results show that CGH is 
      a sensitive method for the detection of all prognostically relevant genetic 
      alterations in neuroblastomas; that CGH considerably simplifies the detection of 
      these alterations, resulting in a single methodological approach; and that CGH is 
      a powerful tool to elucidate previously unknown genetic changes in 
      neuroblastomas.
FAU - Brinkschmidt, C
AU  - Brinkschmidt C
AD  - Gerhard-Domagk-Institute of Pathology, University of Munster, Germany. 
      brinkch@uni-muenster.de
FAU - Christiansen, H
AU  - Christiansen H
FAU - Terpe, H J
AU  - Terpe HJ
FAU - Simon, R
AU  - Simon R
FAU - Boecker, W
AU  - Boecker W
FAU - Lampert, F
AU  - Lampert F
FAU - Stoerkel, S
AU  - Stoerkel S
LA  - eng
PT  - Journal Article
PL  - England
TA  - J Pathol
JT  - The Journal of pathology
JID - 0204634
RN  - 0 (DNA, Neoplasm)
SB  - IM
MH  - Blotting, Southern
MH  - Child
MH  - Child, Preschool
MH  - *Chromosome Aberrations
MH  - Chromosome Deletion
MH  - Chromosomes, Human, Pair 17
MH  - DNA, Neoplasm/*genetics
MH  - Feasibility Studies
MH  - Humans
MH  - Infant
MH  - Neuroblastoma/*genetics/pathology
MH  - Nucleic Acid Hybridization
MH  - Prognosis
EDAT- 1997/04/01 00:00
MHDA- 2000/06/20 09:00
CRDT- 1997/04/01 00:00
PHST- 1997/04/01 00:00 [pubmed]
PHST- 2000/06/20 09:00 [medline]
PHST- 1997/04/01 00:00 [entrez]
AID - 10.1002/(SICI)1096-9896(199704)181:4<394::AID-PATH800>3.0.CO;2-1 [pii]
AID - 10.1002/(SICI)1096-9896(199704)181:4<394::AID-PATH800>3.0.CO;2-1 [doi]
PST - ppublish
SO  - J Pathol. 1997 Apr;181(4):394-400. doi: 
      10.1002/(SICI)1096-9896(199704)181:4<394::AID-PATH800>3.0.CO;2-1.