PMID- 9198360
OWN - NLM
STAT- MEDLINE
DCOM- 19970908
LR  - 20110801
IS  - 0385-2385 (Print)
IS  - 0385-2385 (Linking)
VI  - 39
IP  - 4
DP  - 1997 May
TI  - [Renal lesions of rats induced by antibodies to cultured rat glomerular 
      epithelial cells and mesangial cells].
PG  - 387-94
AB  - Renal lesions of rats induced by antibodies to cultured rat glomerular epithelial 
      cells (GEC) and mesangial cells (GMC) were studied. Antibodies to cultured rat 
      GEC and GMC were produced by immunization of rabbits with GEC and GMC 
      respectively. The rats injected with anti-GEC antibody showed a small amount of 
      urinary protein, glomerular deposition of rabbit IgG and subepithelial dense 
      deposits observed by electron microscopy. Proteinuria was suppressed in the rats 
      in which complement was depleted with cobra venom factor. Western blot analysis 
      demonstrated a band of 200 KD in GEC lysates reacted with anti-GEC antibody. The 
      rats injected with anti-GMC antibody showed little urinary protein, glomerular 
      deposition of rabbit IgG and mesangial dense deposits. Western blot analysis 
      demonstrated two bands of 43 and 14 KD in GMC lysates reacted with anti-GMC 
      antibody. These data showed that the injection of anti-GEC antibody or anti-GMC 
      antibody induced similar lesions to passive Heymann nephritis and anti Thy 1.1 
      nephritis. This study suggested that antigenicity of structural glomerular cells 
      is important as the antigens of glomerulonephritis.
FAU - Tamura, N
AU  - Tamura N
AD  - Department of Internal Medicine II, Hirosaki University, School of Medicusse, 
      Aomori, Japan.
FAU - Yamabe, H
AU  - Yamabe H
FAU - Osawa, H
AU  - Osawa H
FAU - Inuma, H
AU  - Inuma H
FAU - Onodera, K
AU  - Onodera K
LA  - jpn
PT  - English Abstract
PT  - Journal Article
PL  - Japan
TA  - Nihon Jinzo Gakkai Shi
JT  - Nihon Jinzo Gakkai shi
JID - 7505731
RN  - 0 (Autoantibodies)
SB  - IM
MH  - Animals
MH  - *Autoantibodies
MH  - Cells, Cultured
MH  - Glomerular Mesangium/cytology/*immunology
MH  - Glomerulonephritis/*etiology
MH  - Kidney Glomerulus/cytology/*immunology
MH  - Male
MH  - Rabbits
MH  - Rats
MH  - Rats, Sprague-Dawley
EDAT- 1997/05/01 00:00
MHDA- 1997/05/01 00:01
CRDT- 1997/05/01 00:00
PHST- 1997/05/01 00:00 [pubmed]
PHST- 1997/05/01 00:01 [medline]
PHST- 1997/05/01 00:00 [entrez]
PST - ppublish
SO  - Nihon Jinzo Gakkai Shi. 1997 May;39(4):387-94.