PMID- 9199876
OWN - NLM
STAT- MEDLINE
DCOM- 19970822
LR  - 20141120
IS  - 1043-4666 (Print)
IS  - 1043-4666 (Linking)
VI  - 9
IP  - 6
DP  - 1997 Jun
TI  - T cell subsets and cytokines in allergic and non-allergic children. I. Analysis 
      of IL-4, IFN-gamma and IL-13 mRNA expression and protein production.
PG  - 416-26
AB  - Interleukin 4 (IL-4) and IL-13 are key cytokines inducing switching to 
      immunoglobulin E (IgE), whereas interferon gamma (IFN-gamma) acts inhibitory on 
      this process. We analysed whether differences existed in IL-4, IFN-gamma and 
      IL-13 mRNA expression and protein production between T cells of children with 
      allergic and non-allergic asthma, atopic dermatitis and health control children. 
      IL-4 mRNA expression was increased in stimulated T cells of children with 
      allergic asthma and atopic dermatitis, but not in those with non-allergic asthma 
      as compared with healthy controls. Thus the increase in IL-4 expression can be 
      considered as an underlying mechanism of the allergic disease process and not so 
      much of the asthmatic state of the children. In unstimulated T cells of children 
      with atopic dermatitis increased IFN-gamma mRNA expression with a reduced 
      IFN-gamma protein production was found, indicating a post-translational defect in 
      IFN-gamma. Differences in IL-13 expression between the groups were not 
      significant, but IL-13 was significantly correlated with the height of the 
      radio-allergo-sorbent test (RAST) class and with the severity scoring of atopic 
      dermatitis (SCORAD) index. This indicates the clinical relevance of IL-13 for the 
      degree of allergen-specific sensitization and severity of atopic dermatitis. In 
      conclusion, the imbalance in IL-4 and IFN-gamma secretion in patients with atopic 
      dermatitis may reflect general T cell activation in the presence of an intrinsic 
      defect of IFN-gamma secretion.
FAU - Koning, H
AU  - Koning H
AD  - Department of Paediatrics, Sophia Children's Hospital; Rotterdam, The 
      Netherlands.
FAU - Neijens, H J
AU  - Neijens HJ
FAU - Baert, M R
AU  - Baert MR
FAU - Oranje, A P
AU  - Oranje AP
FAU - Savelkoul, H F
AU  - Savelkoul HF
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Cytokine
JT  - Cytokine
JID - 9005353
RN  - 0 (Cytokines)
RN  - 0 (DNA Primers)
RN  - 0 (Interleukin-13)
RN  - 0 (RNA, Messenger)
RN  - 207137-56-2 (Interleukin-4)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Asthma/genetics/immunology/metabolism
MH  - Base Sequence
MH  - Case-Control Studies
MH  - Child, Preschool
MH  - Cytokines/*biosynthesis/*genetics
MH  - DNA Primers/genetics
MH  - Dermatitis, Atopic/genetics/immunology/metabolism
MH  - Gene Expression
MH  - Humans
MH  - Hypersensitivity/*genetics/*immunology
MH  - Immunoglobulin E/biosynthesis/blood
MH  - In Vitro Techniques
MH  - Infant
MH  - Interferon-gamma/biosynthesis/genetics
MH  - Interleukin-13/biosynthesis/genetics
MH  - Interleukin-4/biosynthesis/genetics
MH  - Polymerase Chain Reaction
MH  - RNA, Messenger/genetics/metabolism
MH  - T-Lymphocyte Subsets/*immunology/metabolism
EDAT- 1997/06/01 00:00
MHDA- 1997/06/01 00:01
CRDT- 1997/06/01 00:00
PHST- 1997/06/01 00:00 [pubmed]
PHST- 1997/06/01 00:01 [medline]
PHST- 1997/06/01 00:00 [entrez]
AID - S1043-4666(96)90184-2 [pii]
AID - 10.1006/cyto.1996.0184 [doi]
PST - ppublish
SO  - Cytokine. 1997 Jun;9(6):416-26. doi: 10.1006/cyto.1996.0184.