PMID- 9199877
OWN - NLM
STAT- MEDLINE
DCOM- 19970822
LR  - 20191102
IS  - 1043-4666 (Print)
IS  - 1043-4666 (Linking)
VI  - 9
IP  - 6
DP  - 1997 Jun
TI  - T cells subsets and cytokines in allergic and non-allergic children. II. Analysis 
      and IL-5 and IL-10 mRNA expression and protein production.
PG  - 427-36
AB  - Interleukin 5 (IL-5) has an enhancing effect on IL-4 induced immunoglobulin E 
      (IgE) synthesis. Furthermore, IL-5 plays an important role in the 
      differentiation, recruitment, activation and survival of eosinophils. IL-10 has a 
      downmodulating effect on interferon gamma (IFN-gamma) production and can exert 
      strong anti-inflammatory activities. Therefore, we analysed whether differences 
      were present in IL-5 and IL-10 mRNA expression and protein production between T 
      cells of children with allergic and non-allergic asthma, atopic dermatitis and 
      healthy control children. We demonstrated significant increases in IL-5 mRNA 
      expression and protein production in different T cell fractions of children with 
      allergic and non-allergic asthma and children with atopic dermatitis as compared 
      to healthy controls. This indicates that IL-5 is not only involved in allergy, 
      but also plays a role in the inflammatory process of non-allergic asthma. 
      Interestingly, IL-10 mRNA expression by purified T cells of children with 
      allergic and non-allergic asthma and children with atopic dermatitis was strongly 
      decreased as compared with that of healthy controls. In the peripheral blood 
      mononuclear cell (PBMC) fraction, IL-10 mRNA expression was comparable between 
      the four groups. We hypothesize that this decreased T cell derived IL-10 
      expression results in a lack of immunosuppression of the inflammatory process in 
      these diseases. However, a role of monocyte derived IL-10 cannot be ruled out.
FAU - Koning, H
AU  - Koning H
AD  - Department of Paediatrics, Sophia Children's Hospital; Rotterdam, The 
      Netherlands.
FAU - Neijens, H J
AU  - Neijens HJ
FAU - Baert, M R
AU  - Baert MR
FAU - Oranje, A P
AU  - Oranje AP
FAU - Savelkoul, H F
AU  - Savelkoul HF
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Cytokine
JT  - Cytokine
JID - 9005353
RN  - 0 (Cytokines)
RN  - 0 (DNA Primers)
RN  - 0 (Interleukin-5)
RN  - 0 (RNA, Messenger)
RN  - 130068-27-8 (Interleukin-10)
SB  - IM
MH  - Asthma/genetics/immunology/metabolism
MH  - Base Sequence
MH  - Case-Control Studies
MH  - Child, Preschool
MH  - Cytokines/*biosynthesis/*genetics
MH  - DNA Primers/genetics
MH  - Dermatitis, Atopic/genetics/immunology/metabolism
MH  - Eosinophils
MH  - Gene Expression
MH  - Humans
MH  - Hypersensitivity/*genetics/*immunology/metabolism
MH  - In Vitro Techniques
MH  - Infant
MH  - Interleukin-10/*biosynthesis/*genetics
MH  - Interleukin-5/*biosynthesis/*genetics
MH  - Leukocyte Count
MH  - Polymerase Chain Reaction
MH  - RNA, Messenger/genetics/metabolism
MH  - T-Lymphocyte Subsets/*immunology/metabolism
EDAT- 1997/06/01 00:00
MHDA- 1997/06/01 00:01
CRDT- 1997/06/01 00:00
PHST- 1997/06/01 00:00 [pubmed]
PHST- 1997/06/01 00:01 [medline]
PHST- 1997/06/01 00:00 [entrez]
AID - S1043466696901854 [pii]
AID - 10.1006/cyto.1996.0185 [doi]
PST - ppublish
SO  - Cytokine. 1997 Jun;9(6):427-36. doi: 10.1006/cyto.1996.0185.