PMID- 9200479
OWN - NLM
STAT- MEDLINE
DCOM- 19970714
LR  - 20071114
IS  - 0022-1767 (Print)
IS  - 0022-1767 (Linking)
VI  - 159
IP  - 1
DP  - 1997 Jul 1
TI  - Transgenic monocyte chemoattractant protein-1 (MCP-1) in pancreatic islets 
      produces monocyte-rich insulitis without diabetes: abrogation by a second 
      transgene expressing systemic MCP-1.
PG  - 401-8
AB  - Monocyte chemoattractant protein-1 (MCP-1) is a CC chemokine that attracts 
      monocytes and T lymphocytes in vitro; however, its in vivo functions are poorly 
      understood. To address this question, we constructed transgenic mice expressing 
      MCP-1 controlled by an insulin promoter. These mice developed a chronic insulitic 
      infiltrate composed of F4/80+ monocytes with minor populations of CD4+, CD8+, and 
      B220+ cells. Despite persistent transgene expression, the insulitis never 
      progressed, and blood glucose levels remained normal. Thus, MCP-1 alone is 
      sufficient to elicit a monocytic infiltrate, but not to activate elicited cells. 
      These results differ from those obtained with another transgenic model using the 
      mouse mammary tumor virus long terminal repeat, in which mice expressed 
      substantial MCP-1 in several organs but had no infiltrates. However, mice 
      expressing both transgenes had minimal insulitis, indicating that high systemic 
      levels of MCP-1 prevented monocytes from responding to local MCP-1. Thus, the 
      ability of MCP-1 to elicit monocytic infiltration depends on its being expressed 
      at low levels in an anatomically restricted area.
FAU - Grewal, I S
AU  - Grewal IS
AD  - Section of Immunobiology, Yale University School of Medicine, New Haven, CT 
      06510, USA.
FAU - Rutledge, B J
AU  - Rutledge BJ
FAU - Fiorillo, J A
AU  - Fiorillo JA
FAU - Gu, L
AU  - Gu L
FAU - Gladue, R P
AU  - Gladue RP
FAU - Flavell, R A
AU  - Flavell RA
FAU - Rollins, B J
AU  - Rollins BJ
LA  - eng
GR  - CA53091/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (Chemokine CCL2)
SB  - IM
MH  - Animals
MH  - Cell Movement
MH  - Chemokine CCL2/*biosynthesis/genetics
MH  - Gene Transfer Techniques
MH  - Islets of Langerhans/*immunology/metabolism/pathology
MH  - Mice
MH  - Mice, Transgenic
MH  - Monocytes/*immunology/pathology
MH  - Pancreatic Diseases/genetics/*immunology/metabolism
EDAT- 1997/07/01 00:00
MHDA- 1997/07/01 00:01
CRDT- 1997/07/01 00:00
PHST- 1997/07/01 00:00 [pubmed]
PHST- 1997/07/01 00:01 [medline]
PHST- 1997/07/01 00:00 [entrez]
PST - ppublish
SO  - J Immunol. 1997 Jul 1;159(1):401-8.